Aerobic waste activated sludge (WAS) for start-up seed of mesophilic and thermophilic anaerobic digestion

Since there are very limited numbers of thermophilic anaerobic digesters being operated, it is often difficult to start up a new one using sludge from an existing reactor as a seed. However, for obvious reasons it seems few attempts have been made to compare the start-up performance of thermophilic anaerobic digestion using different sources of seed sludges. The purpose of this study was to evaluate the start-up performance of anaerobic digestion using aerobic waste activated sludge (WAS) from a plant which has no anaerobic digesters and mesophilic anaerobic digested sludge (ADS) as the seed source at both mesophilic (35°C) and thermophilic (55°C) temperatures. In this study, two experiments were conducted. First, thermophilic anaerobic reactors were seeded with WAS (VSS=4400 mg/L) and ADS (VSS=14,500 mg/L) to investigate start-up performance with a feed of acetate as well as propionate. The results show that WAS started to produce CH 4 soon after acetate feeding without a lag time, while ADS had a lag time of 10 days. When the feed was changed to propionate, WAS removed propionate down to below the detection limit of 10 mg/L, while ADS removed little propionate and produced little CH 4. Second, in order to further compare the methanogenic activity of WAS and ADS, both mesophilic and thermophilic reactors were operated. WAS acclimated to anaerobic conditions shortly (