Evaluation of the potential of Delta-aminolevulinic acid for simultaneous detection of bioburden and anti-microbial photodynamic therapy of MRSA infected wounds in Swiss albino mice

The dramatic increase of drug-resistant bacteria necessitates urgent development of platforms to simultaneously detect and inactivate bacteria causing wound infections, but are confronted with various challenges. Delta amino levulinic acid (ALA) induced protoporphyrin IX (PpIX) can be a promising modality for simultaneous bioburden diagnostics and therapeutics. Herein, we report utility of ALA induced protoporphyrin (PpIX) based simultaneous bioburden detection, photoinactivation and therapeutic outcome assessment in methicillin resistant Staphylococcus aureus (MRSA) infected wounds of mice. MRSA infected wounds treated with 10% ALA were imaged with help of a blue LED (∼405 nm) based, USB powered, hand held device integrated with a modular graphic user interface (GUI). Effect of ALA application time, bacteria load, post bacteria application time points on wound fluorescence studied. PpIX fluorescence observed after excitation with blue LEDs was used to detect bioburden, start red light mediated antimicrobial photodynamic therapy (aPDT), determine aPDT effectiveness and assess selectivity of the approach. ALA-PpIX fluorescence of wound bed discriminates infected from uninfected wounds and detects clinically relevant load. While wound fluorescence pattern changes as a function of ALA incubation and post infection time, intra-wound inhomogeneity in fluorescence correlates with the Gram staining data on presence of biofilms foci. Lack of red fluorescence from wound granulation tissue treated with ALA suggests selectivity of the approach. Further, significant reduction (∼50%) in red fluorescence, quantified using the GUI, relates well with bacteria load reduction observed post topical aPDT. The potential of ALA induced PpIX for simultaneous detection of bioburden, photodynamic inactivation and “florescence-guided aPDT assessment” is demonstrated in MRSA infected wounds of mice. Potential utility of ALA induced PpIX to detect bioburden, exert anti microbial photodynamic therapy (aPDT) and assess therapeutic outcome in MRSA infected wounds of mice. The hand-held device fitted with four 405 nm LEDs and a camera was used for excitation of PpIX and fluorescence imaging, respectively. aPDT induced reactive oxygen species (ROS) leads to bacteria inactivation and loss of fluorescence (white arrows). [Display omitted] •δ -ALA-PpIX for wound infection diagnosis and aPDT.•Florescence detection using a LED based, hand held device.•Red light excitation based aPDT.•Fluoresce