Evaluation of PDTC for removing Ni(II) from human serum transferrin in vitro

Pyrrolidine dithiocarbamate (PDTC) can be an oral chelator with pl(a= 3.300d:0.00 . It behaves as a bidentate ligand at serum pH. The effect of pH on Ni2+-Tf indicated that the maxim m adsorption was at pH=7.4. The effective Ni-PDTC binding constant was determined (logk=11.1 .1) for the 1:2 Ni(PDTC)2 complex using UV-vis spectra. The isosbestic point at 298 indicated that he complexation reaction was done directly (without side reaction). Removal of Ni from transferrin TO was investigated by reverse titration of PDTC at 25 deg C and pH=7.4 using UV-vis spectra. PDT is able to remove 25% of Ni from human serum transferrin.