A new perspective on structural characterisation and immunomodulatory activity of arabinogalactan in Larix kaempferi from Qinling Mountains

In this study, crude polysaccharide (LAG-C) and homogeneous arabinogalactan (LAG-W) were isolated from Qinling Larix kaempferi of Shaanxi Province. Bioactivity assays showed that LAG-W and LAG-C enhanced the phagocytic ability, NO secretion, acid phosphatase activity, and cytokine production (IL-6,...

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Veröffentlicht in:International journal of biological macromolecules 2024-04, Vol.265 (Pt 2), p.130859-130859, Article 130859
Hauptverfasser: Huang, Wenqi, Xie, Yutao, Guo, Tongyi, Dai, Wei, Nan, Linhua, Wang, Qian, Liu, Yuxia, Lan, Wenxian, Wang, Zhongfu, Huang, Linjuan, Gong, Guiping
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Sprache:eng
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Zusammenfassung:In this study, crude polysaccharide (LAG-C) and homogeneous arabinogalactan (LAG-W) were isolated from Qinling Larix kaempferi of Shaanxi Province. Bioactivity assays showed that LAG-W and LAG-C enhanced the phagocytic ability, NO secretion, acid phosphatase activity, and cytokine production (IL-6, IL-1β, and TNF-α) of RAW264.7 macrophages. Notably, LAG-W exhibited a significantly stronger immunomodulatory effect than LAG-C. The primary structure of LAG-W was characterised by chemical methods (monosaccharide composition, methylation analysis, and alkali treatment) and spectroscopic techniques (gas chromatography–mass spectrometry, high-performance liquid chromatography-mass spectrometry, and 1D/2D nuclear magnetic resonance). LAG-W was identified as a 22.08 kilodaltons (kDa) neutral polysaccharide composed of arabinose and galactose at a 1:7.5 molar ratio. Its backbone consisted of repeated →3)-β-Galp-(1→ residues. Side chains, connected at the O-6 position, were mainly composed of T-β-Galp-(1→ and T-β-Galp-(1→6)-β-Galp-(1→ residues. And it also contained small amounts of T-β-Arap-(1→, T-α-Araf-(1→6)-β-Galp-(1→6)-β-Galp-(1→, and T-α-Araf-(1→3)-α-Araf-(1→6)-β-Galp-(1→ residues. By structurally and functionally characterising L. kaempferi polysaccharides, this study opens the way for the valorisation of this species.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2024.130859