New substrate sample handling and micro-Raman sensing methods for biomolecular analysis

The paper discusses a novel sample deposition method and a new substrate for micro-Raman applications, together with some applications for pharmaceutical and fluorescent compound analysis. Teophylline and indigo carmine azo-dye were used as testing molecules. They were able to be detected in trace amounts, compatible with the applications at physiological level. SERS samples of Ag colloid containing small amounts of tested molecule solutions were drop-coated onto distinct small regions of the SpectRiM plate and characterized using AFM and micro-Raman spectroscopy. An enhancement mechanism from the first monolayer of drop coated deposition SERS sample was concluded.