Enzyme Immobilization on Porous Silicon Surfaces

Enzyme Immobilization on Porous Silicon Surfaces

β‐Glucuronidase enzymes have been attached to a porous silicon surface through a direct silicon–carbon bond based linking system (see Figure). The attached enzymes display high activity and the photoluminescent (PL) properties and surface stability of the porous silicon are retained. Quenching of th...

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Veröffentlicht in:Advanced materials (Weinheim) 2004-04, Vol.16 (8), p.689-693
Hauptverfasser: Létant, S. E., Hart, B. R., Kane, S. R., Hadi, M. Z., Shields, S. J., Reynolds, J. G.
Format: Artikel
Sprache:eng
Schlagworte:
bio
Enzymes
porous
Porous materials
Sensors
Sensors, bio
Silicon
Silicon, porous
Transducers
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Zusammenfassung:β‐Glucuronidase enzymes have been attached to a porous silicon surface through a direct silicon–carbon bond based linking system (see Figure). The attached enzymes display high activity and the photoluminescent (PL) properties and surface stability of the porous silicon are retained. Quenching of the PL is observed upon enzymatic breakdown of the substrate, leading to the possibility of a new class of chemical and biological sensors.
ISSN:0935-9648
1521-4095
DOI:10.1002/adma.200306173