The anti-apoptotic role of Ginkgolide B via mitochondrial permeability transition pore inhibition in retinal ischemia-reperfusion

This research delves into the effectiveness of Ginkgolide B (GB), a compound from Ginkgo biloba, in combating cell death caused by glaucoma, with a focus on mitochondrial impairment and the mitochondrial permeability transition pore (mPTP). Utilizing models of high intraocular pressure and in vitro...

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Veröffentlicht in:Biochemical and biophysical research communications 2024-04, Vol.705, p.149722, Article 149722
Hauptverfasser: Wang, Xiou, Hu, Baoqi, Zhang, Hanyue, Zhou, Sijia, Wu, Wenfei, Tan, Zizhu, Sun, Meiqi, Kang, Qianyan
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Sprache:eng
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Zusammenfassung:This research delves into the effectiveness of Ginkgolide B (GB), a compound from Ginkgo biloba, in combating cell death caused by glaucoma, with a focus on mitochondrial impairment and the mitochondrial permeability transition pore (mPTP). Utilizing models of high intraocular pressure and in vitro glaucoma simulations, the study investigates GB's impact on retinal progenitor cells (RPCs) under oxygen-glucose deprivation/reperfusion (OGD/R) and in a rat glaucoma model. The study methodologies included apoptosis assessment, apoptotic marker analysis via Western blot, and mitochondrial structure and function evaluation. The findings reveal that GB notably decreases apoptosis in RPCs exposed to OGD/R in vitro, and reduces ischemia-reperfusion damage in vivo. GB's protective role is attributed to its ability to preserve mitochondrial integrity, maintain membrane potential, regulate calcium levels, and inhibit mPTP opening. These results underscore GB's potential as a therapeutic agent for acute primary angle-closure glaucoma, highlighting its capability to alleviate mitochondrial damage and apoptosis in RPCs and retinal nerve fiber layer cells. •Ginkgolide B can reduce OGD/R indued Retinal Progenitor Cells' apoptosis.•Ginkgolide B impacts on Retinal Progenitor Cells via inhibiting the opening of Mitochondrial Permeability Transition Pore.•GB is expected to be a protective agent against retinal ischemia-reperfusion injury by attenuating apoptotic damage in RNFL.
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2024.149722