Cell-free translation system with artificial lipid-monolayer particles as a unique tool for characterizing lipid-monolayer binding proteins

Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2024-04, Vol.88 (5), p.555-560
Hauptverfasser:	Kuroiwa, Fu, Suda, Hiraku, Yabuki, Maho, Atsuzawa, Kimie, Yamaguchi, Haruhiko, Toyota, Masatsugu, Kaneko, Yasuko, Yamashita, Satoshi, Takahashi, Seiji, Tozawa, Yuzuru
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell-Free System Lipids - chemistry Protein Binding Protein Biosynthesis Proteins - chemistry Proteins - metabolism
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Methods for functional analysis of proteins specifically localizing to lipid monolayers such as rubber particles and lipid droplets are limited. We have succeeded in establishing a system in which artificially prepared lipid monolayer particles are added to a cell-free translation system to confirm the properties of proteins that specifically bind to lipid monolayers in a translation-coupled manner.
ISSN:	1347-6947 1347-6947
DOI:	10.1093/bbb/zbae026