Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice

Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of agricultural and food chemistry 2024-03, Vol.72 (9), p.4801-4813
Hauptverfasser: Xu, Qin, Wang, Jianing, Zhang, Yi, Li, Ying, Qin, Xinshu, Xin, Yirao, Li, Yinglei, Xu, Ke, Yang, Xingbin, Wang, Xingyu
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 4813
container_issue 9
container_start_page 4801
container_title Journal of agricultural and food chemistry
container_volume 72
creator Xu, Qin
Wang, Jianing
Zhang, Yi
Li, Ying
Qin, Xinshu
Xin, Yirao
Li, Yinglei
Xu, Ke
Yang, Xingbin
Wang, Xingyu
description Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.
doi_str_mv 10.1021/acs.jafc.3c09511
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2938282917</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2938282917</sourcerecordid><originalsourceid>FETCH-LOGICAL-a336t-3618ae40e4b90164d66b163fa91dd8c24d28c1d3d644a8cb33c4b32d3e9307413</originalsourceid><addsrcrecordid>eNp1kMFPwjAYxRujEUTvnkyPHhy2-8ZovSGCmqAS0PPStR0p2VZcuwNX_3KLoDdP_dr-3sv3HkKXlPQpiemtkK6_FoXsgyR8QOkR6tJBTKIwsmPUJYGJ2CClHXTm3JoQwgZDcoo6wIAD59BFXyO_3RgpSjwvRe1xZRavIxzuUZhiTukdXti8dR4vvchNafwWi5UwdXiZWqvwg1lp542tsagVXm60NIWReN7YynpTr_CkKLT0OAD34UfZXEivG9NW2NT4xUh9jk4KUTp9cTh76GM6eR8_RbO3x-fxaBYJgNRHkFImdEJ0knNC00SlaU5TKASnSjEZJypmkipQaZIIJnMAmeQQK9AcyDCh0EPXe99NYz_bsHRWGSd1GXJr27os5sBiFjIPA0r2qGysc40usk1jKtFsM0qyXfNZaD7bNZ8dmg-Sq4N7m1da_Ql-qw7AzR74kdq2qUPY__2-ATLzjuA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2938282917</pqid></control><display><type>article</type><title>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Xu, Qin ; Wang, Jianing ; Zhang, Yi ; Li, Ying ; Qin, Xinshu ; Xin, Yirao ; Li, Yinglei ; Xu, Ke ; Yang, Xingbin ; Wang, Xingyu</creator><creatorcontrib>Xu, Qin ; Wang, Jianing ; Zhang, Yi ; Li, Ying ; Qin, Xinshu ; Xin, Yirao ; Li, Yinglei ; Xu, Ke ; Yang, Xingbin ; Wang, Xingyu</creatorcontrib><description>Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.</description><identifier>ISSN: 0021-8561</identifier><identifier>ISSN: 1520-5118</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/acs.jafc.3c09511</identifier><identifier>PMID: 38393993</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Bifidobacterium - genetics ; Bifidobacterium - metabolism ; Bioactive Constituents, Metabolites, and Functions ; Digestion ; Food ; Gastrointestinal Microbiome ; Mice ; MicroRNAs - genetics ; MicroRNAs - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2024-03, Vol.72 (9), p.4801-4813</ispartof><rights>2024 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a336t-3618ae40e4b90164d66b163fa91dd8c24d28c1d3d644a8cb33c4b32d3e9307413</citedby><cites>FETCH-LOGICAL-a336t-3618ae40e4b90164d66b163fa91dd8c24d28c1d3d644a8cb33c4b32d3e9307413</cites><orcidid>0000-0002-8039-0525 ; 0000-0003-4000-464X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.jafc.3c09511$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.jafc.3c09511$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38393993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Qin</creatorcontrib><creatorcontrib>Wang, Jianing</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Qin, Xinshu</creatorcontrib><creatorcontrib>Xin, Yirao</creatorcontrib><creatorcontrib>Li, Yinglei</creatorcontrib><creatorcontrib>Xu, Ke</creatorcontrib><creatorcontrib>Yang, Xingbin</creatorcontrib><creatorcontrib>Wang, Xingyu</creatorcontrib><title>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.</description><subject>Animals</subject><subject>Bifidobacterium - genetics</subject><subject>Bifidobacterium - metabolism</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Digestion</subject><subject>Food</subject><subject>Gastrointestinal Microbiome</subject><subject>Mice</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFPwjAYxRujEUTvnkyPHhy2-8ZovSGCmqAS0PPStR0p2VZcuwNX_3KLoDdP_dr-3sv3HkKXlPQpiemtkK6_FoXsgyR8QOkR6tJBTKIwsmPUJYGJ2CClHXTm3JoQwgZDcoo6wIAD59BFXyO_3RgpSjwvRe1xZRavIxzuUZhiTukdXti8dR4vvchNafwWi5UwdXiZWqvwg1lp542tsagVXm60NIWReN7YynpTr_CkKLT0OAD34UfZXEivG9NW2NT4xUh9jk4KUTp9cTh76GM6eR8_RbO3x-fxaBYJgNRHkFImdEJ0knNC00SlaU5TKASnSjEZJypmkipQaZIIJnMAmeQQK9AcyDCh0EPXe99NYz_bsHRWGSd1GXJr27os5sBiFjIPA0r2qGysc40usk1jKtFsM0qyXfNZaD7bNZ8dmg-Sq4N7m1da_Ql-qw7AzR74kdq2qUPY__2-ATLzjuA</recordid><startdate>20240306</startdate><enddate>20240306</enddate><creator>Xu, Qin</creator><creator>Wang, Jianing</creator><creator>Zhang, Yi</creator><creator>Li, Ying</creator><creator>Qin, Xinshu</creator><creator>Xin, Yirao</creator><creator>Li, Yinglei</creator><creator>Xu, Ke</creator><creator>Yang, Xingbin</creator><creator>Wang, Xingyu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid><orcidid>https://orcid.org/0000-0003-4000-464X</orcidid></search><sort><creationdate>20240306</creationdate><title>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</title><author>Xu, Qin ; Wang, Jianing ; Zhang, Yi ; Li, Ying ; Qin, Xinshu ; Xin, Yirao ; Li, Yinglei ; Xu, Ke ; Yang, Xingbin ; Wang, Xingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-3618ae40e4b90164d66b163fa91dd8c24d28c1d3d644a8cb33c4b32d3e9307413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bifidobacterium - genetics</topic><topic>Bifidobacterium - metabolism</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Digestion</topic><topic>Food</topic><topic>Gastrointestinal Microbiome</topic><topic>Mice</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Qin</creatorcontrib><creatorcontrib>Wang, Jianing</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Qin, Xinshu</creatorcontrib><creatorcontrib>Xin, Yirao</creatorcontrib><creatorcontrib>Li, Yinglei</creatorcontrib><creatorcontrib>Xu, Ke</creatorcontrib><creatorcontrib>Yang, Xingbin</creatorcontrib><creatorcontrib>Wang, Xingyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Qin</au><au>Wang, Jianing</au><au>Zhang, Yi</au><au>Li, Ying</au><au>Qin, Xinshu</au><au>Xin, Yirao</au><au>Li, Yinglei</au><au>Xu, Ke</au><au>Yang, Xingbin</au><au>Wang, Xingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2024-03-06</date><risdate>2024</risdate><volume>72</volume><issue>9</issue><spage>4801</spage><epage>4813</epage><pages>4801-4813</pages><issn>0021-8561</issn><issn>1520-5118</issn><eissn>1520-5118</eissn><abstract>Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38393993</pmid><doi>10.1021/acs.jafc.3c09511</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid><orcidid>https://orcid.org/0000-0003-4000-464X</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0021-8561
ispartof Journal of agricultural and food chemistry, 2024-03, Vol.72 (9), p.4801-4813
issn 0021-8561
1520-5118
1520-5118
language eng
recordid cdi_proquest_miscellaneous_2938282917
source MEDLINE; American Chemical Society Journals
subjects Animals
Bifidobacterium - genetics
Bifidobacterium - metabolism
Bioactive Constituents, Metabolites, and Functions
Digestion
Food
Gastrointestinal Microbiome
Mice
MicroRNAs - genetics
MicroRNAs - metabolism
title Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T07%3A23%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Atypical%20Plant%20miRNA%20cal-miR2911:%20Robust%20Stability%20against%20Food%20Digestion%20and%20Specific%20Promoting%20Effect%20on%20Bifidobacterium%20in%20Mice&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Xu,%20Qin&rft.date=2024-03-06&rft.volume=72&rft.issue=9&rft.spage=4801&rft.epage=4813&rft.pages=4801-4813&rft.issn=0021-8561&rft.eissn=1520-5118&rft_id=info:doi/10.1021/acs.jafc.3c09511&rft_dat=%3Cproquest_cross%3E2938282917%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2938282917&rft_id=info:pmid/38393993&rfr_iscdi=true