Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice
Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in...
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Veröffentlicht in: | Journal of agricultural and food chemistry 2024-03, Vol.72 (9), p.4801-4813 |
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creator | Xu, Qin Wang, Jianing Zhang, Yi Li, Ying Qin, Xinshu Xin, Yirao Li, Yinglei Xu, Ke Yang, Xingbin Wang, Xingyu |
description | Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising. |
doi_str_mv | 10.1021/acs.jafc.3c09511 |
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Agric. Food Chem</addtitle><description>Previous studies showed that cal-miR2911, featuring an atypical biogenesis, could target genes of virus and in turn inhibit virus replication. Given its especial sequence motif and cross-kingdom potential, the stability of miR2911 under digestive environment and its impact on intestinal microbes in mice were examined. The results showed that miR2911 was of considerable stability during oral, gastric, and intestinal digestion. The coingested food matrix enhanced its stability in the gastric phase, contributing to the existence of miR2911 in mouse intestines. The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.</description><subject>Animals</subject><subject>Bifidobacterium - genetics</subject><subject>Bifidobacterium - metabolism</subject><subject>Bioactive Constituents, Metabolites, and Functions</subject><subject>Digestion</subject><subject>Food</subject><subject>Gastrointestinal Microbiome</subject><subject>Mice</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFPwjAYxRujEUTvnkyPHhy2-8ZovSGCmqAS0PPStR0p2VZcuwNX_3KLoDdP_dr-3sv3HkKXlPQpiemtkK6_FoXsgyR8QOkR6tJBTKIwsmPUJYGJ2CClHXTm3JoQwgZDcoo6wIAD59BFXyO_3RgpSjwvRe1xZRavIxzuUZhiTukdXti8dR4vvchNafwWi5UwdXiZWqvwg1lp542tsagVXm60NIWReN7YynpTr_CkKLT0OAD34UfZXEivG9NW2NT4xUh9jk4KUTp9cTh76GM6eR8_RbO3x-fxaBYJgNRHkFImdEJ0knNC00SlaU5TKASnSjEZJypmkipQaZIIJnMAmeQQK9AcyDCh0EPXe99NYz_bsHRWGSd1GXJr27os5sBiFjIPA0r2qGysc40usk1jKtFsM0qyXfNZaD7bNZ8dmg-Sq4N7m1da_Ql-qw7AzR74kdq2qUPY__2-ATLzjuA</recordid><startdate>20240306</startdate><enddate>20240306</enddate><creator>Xu, Qin</creator><creator>Wang, Jianing</creator><creator>Zhang, Yi</creator><creator>Li, Ying</creator><creator>Qin, Xinshu</creator><creator>Xin, Yirao</creator><creator>Li, Yinglei</creator><creator>Xu, Ke</creator><creator>Yang, Xingbin</creator><creator>Wang, Xingyu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid><orcidid>https://orcid.org/0000-0003-4000-464X</orcidid></search><sort><creationdate>20240306</creationdate><title>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</title><author>Xu, Qin ; Wang, Jianing ; Zhang, Yi ; Li, Ying ; Qin, Xinshu ; Xin, Yirao ; Li, Yinglei ; Xu, Ke ; Yang, Xingbin ; Wang, Xingyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a336t-3618ae40e4b90164d66b163fa91dd8c24d28c1d3d644a8cb33c4b32d3e9307413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Bifidobacterium - genetics</topic><topic>Bifidobacterium - metabolism</topic><topic>Bioactive Constituents, Metabolites, and Functions</topic><topic>Digestion</topic><topic>Food</topic><topic>Gastrointestinal Microbiome</topic><topic>Mice</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Qin</creatorcontrib><creatorcontrib>Wang, Jianing</creatorcontrib><creatorcontrib>Zhang, Yi</creatorcontrib><creatorcontrib>Li, Ying</creatorcontrib><creatorcontrib>Qin, Xinshu</creatorcontrib><creatorcontrib>Xin, Yirao</creatorcontrib><creatorcontrib>Li, Yinglei</creatorcontrib><creatorcontrib>Xu, Ke</creatorcontrib><creatorcontrib>Yang, Xingbin</creatorcontrib><creatorcontrib>Wang, Xingyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Qin</au><au>Wang, Jianing</au><au>Zhang, Yi</au><au>Li, Ying</au><au>Qin, Xinshu</au><au>Xin, Yirao</au><au>Li, Yinglei</au><au>Xu, Ke</au><au>Yang, Xingbin</au><au>Wang, Xingyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. 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The survival miR2911 promoted the growth of Bifidobacterium in mice and maintained the overall composition and diversity of the gut microbiota. miR2911 specifically entered the cells of Bifidobacterium adolescentis and potentially modulated the gene expression as evidenced by the dual-luciferase assay. The current study provided evidence on the cross-kingdom communication between dietary miRNAs and gut microbes, suggesting that modulating target bacteria using miRNAs for nutritional and therapeutic ends is promising.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38393993</pmid><doi>10.1021/acs.jafc.3c09511</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8039-0525</orcidid><orcidid>https://orcid.org/0000-0003-4000-464X</orcidid></addata></record> |
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subjects | Animals Bifidobacterium - genetics Bifidobacterium - metabolism Bioactive Constituents, Metabolites, and Functions Digestion Food Gastrointestinal Microbiome Mice MicroRNAs - genetics MicroRNAs - metabolism |
title | Atypical Plant miRNA cal-miR2911: Robust Stability against Food Digestion and Specific Promoting Effect on Bifidobacterium in Mice |
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