Influence of increasing anode surface area on nitrite-absent ammonium oxidation in a continuous single-chamber bio-electrochemical system

Reducing energy consumption in conventional nitrogen removal processes is a crucial and urgent requirement. This study proposes an efficient electrode-dependent bio-electrochemical anaerobic ammonium (NH4+-N) oxidation (BE-ANAMMOX) process, employing a carbon brush as the electron acceptor and voltage of 0.8 V. The applied voltage facilitated the removal of NH4+-N with a maximum removal efficiency of 41% and a Coulombic efficiency of 40.92%, without the addition of nitrite (NO2−-N). Furthermore, the NH4+-N removal efficiency demonstrated an increase corresponding to the increase in the anodic surface area. The bio-electrochemical NH4+-N removal achieved remarkable reductions, eliminating the need for O2 and NO2−-N by 100%, lowering energy consumption by 67%, and reducing CO2 emissions by 66% when treating 1 kg of NH4+-N. An analysis of the microbial community revealed an increase in nitrifiers and denitrifiers, including Exiguobacterium aestuarii, Alishewanella aestuarii, Comamonas granuli, and Acinetobacter baumannii. This intricate process involved the direct conversion of NH4+-N to N2 by ANAMMOX bacteria through extracellular electron transfer, all without NO2−-N. Thus, bio-electrochemical NH4+-N removal exhibits promising potential for effective nitrogen removal in wastewater treatment facilities. [Display omitted] •Bio-electrochemical anaerobic NH4+-N oxidation (BE-ANAMMOX) process proposed.•NH4+ oxidized using a carbon brush as the sole electron acceptor with NO2− absent.•ANAMMOX microorganisms possess extracellular electron transfer capabilities.•The electrode-dependent process achieved 41% NH4+-N removal efficiency.•The electrode-dependent process reduced energy consumption and CO2 emissions.