Picea wilsonii NAC31 and DREB2A Cooperatively Activate ERD1 to Modulate Drought Resistance in Transgenic Arabidopsis

The NAC family of transcription factors (TFs) regulate plant development and abiotic stress. However, the specific function and response mechanism of NAC TFs that increase drought resistance in remain largely unknown. In this study, we functionally characterized a member of the PwNAC family known as...

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Veröffentlicht in:International journal of molecular sciences 2024-02, Vol.25 (4), p.2037
Hauptverfasser: Huang, Yiming, Du, Bingshuai, Yu, Mingxin, Cao, Yibo, Liang, Kehao, Zhang, Lingyun
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Sprache:eng
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Zusammenfassung:The NAC family of transcription factors (TFs) regulate plant development and abiotic stress. However, the specific function and response mechanism of NAC TFs that increase drought resistance in remain largely unknown. In this study, we functionally characterized a member of the PwNAC family known as . PwNAC31 is a nuclear-localized protein with transcriptional activation activity and contains an NAC domain that shows extensive homology with ANAC072 in . The expression level of is significantly upregulated under drought and ABA treatments. The heterologous expression of in mutants enhances the seed vigor and germination rates and restores the hypersensitive phenotype of under drought stress, accompanied by the up-regulated expression of drought-responsive genes such as and . Yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PwNAC31 interacts with DREB2A and ABF3 (ABSCISIC ACID-RESPONSIVE ELEMENT-BINDING FACTOR 3). Yeast one-hybrid and dual-luciferase assays showed that PwNAC31, together with its interaction protein DREB2A, directly regulated the expression of by binding to the DRE element of the promoter. Collectively, our study provides evidence that PwNAC31 activates by interacting with DREB2A to enhance drought tolerance in transgenic .
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25042037