Absorption-based colorimetric detection of nickel(II) ion by phase separation of thermoresponsive magnetic nanoparticles under microflow

Therma-Max™ LSA Streptavidin is a thermoresponsive magnetic nanoparticle (TMNP). It can be introduced conveniently to molecular recognition groups by avidin–biotin interaction. In this study, we demonstrated the detection of nickel(II) ions by the magnetic separation of TMNP induced by their phase transition under microflow. The NTA-tagged TMNP solution mixed with a Ni 2+ sample was introduced into a microchannel with a well structure. Moreover, the sample was heated to induce the thermally induced aggregation of TMNP. The Ni-capturing TMNP were trapped in the well by magnetic fields. The supernatant was removed from the outlet, and a dimethylglyoxime (DMG) solution was introduced into the device for colorimetric detection in the well. Because DMG has a higher stability constant with Ni 2+ , sensitive colorimetric detection of Ni 2+ can be achieved in devices where the sample volume, e.g., optical pathlength, is short. To demonstrate the feasibility of the proposed method, a recovery test was conducted using a commercially available cosmetic sample. Therein, complete collection was achieved. Graphical abstract