Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis

Fourier-transform infrared (FTIR) spectroscopy using the IR Biotyper and core genome single nucleotide polymorphism (cgSNP) analysis were performed on 12 Legionella isolates associated with an outbreak at a spa house in Kanagawa Prefecture, Japan, and 3 non-outbreak isolates. The discriminative powe...

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Veröffentlicht in:	European journal of clinical microbiology & infectious diseases 2024-05, Vol.43 (5), p.991-997
Hauptverfasser:	Nakajima, Naoki, Jinnai, Michio, Izumiyama, Shinji, Kuroki, Toshiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Bacterial Typing Techniques - methods Biomedical and Life Sciences Biomedicine Brief Report Disease Outbreaks Epidemics Epidemiology Fourier transforms Genomes Humans Infectious diseases Infrared spectroscopy Internal Medicine Japan - epidemiology Laboratories Legionella Legionella - classification Legionella - genetics Legionella - isolation & purification Legionella pneumophila - classification Legionella pneumophila - genetics Legionella pneumophila - isolation & purification Legionellosis - diagnosis Legionellosis - epidemiology Legionellosis - microbiology Medical Microbiology Methods Nucleotides Outbreaks Polymorphism Polymorphism, Single Nucleotide Public health Single-nucleotide polymorphism Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Typing
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Zusammenfassung:	Fourier-transform infrared (FTIR) spectroscopy using the IR Biotyper and core genome single nucleotide polymorphism (cgSNP) analysis were performed on 12 Legionella isolates associated with an outbreak at a spa house in Kanagawa Prefecture, Japan, and 3 non-outbreak isolates. The discriminative power of FTIR spectroscopy for 48-h incubation conditions of L . pneumophila in this outbreak was lower than cgSNP-based typing but higher than serogroup typing. FTIR spectroscopy could screen outbreak isolates from a group of genetically related isolates and may be useful as an initial typing method in Legionella outbreak investigations.
ISSN:	0934-9723 1435-4373
DOI:	10.1007/s10096-024-04781-3