Fluorochrome‐labeled inhibitors of caspase‐1 require membrane permeabilization to efficiently access caspase‐1 in macrophages

Fluorochrome‐labeled inhibitors of caspase‐1 require membrane permeabilization to efficiently access caspase‐1 in macrophages

Caspase‐1 location in cells has been studied with fluorochrome‐labeled inhibitors of caspase‐1 (FLICA reagents). We report that FLICA reagents have limited cell‐membrane permeability. This impacts experimental design as cells with intact membranes, including caspase‐1 knockout cells, are not appropr...

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Veröffentlicht in:European journal of immunology 2024-05, Vol.54 (5), p.e2350515-n/a
Hauptverfasser: Thygesen, Sara J, Burgener, Sabrina S, Mudai, Prerna, Monteleone, Mercedes, Boucher, Dave, Sagulenko, Vitaliya, Schroder, Kate, Stacey, Katryn J
Format: Artikel
Sprache:eng
Schlagworte:
Caspase
Caspase inhibitor
Caspase‐1
FLICA
Gasdermin D
Inflammasome
Inflammasomes
Macrophages
Membrane permeability
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Zusammenfassung:Caspase‐1 location in cells has been studied with fluorochrome‐labeled inhibitors of caspase‐1 (FLICA reagents). We report that FLICA reagents have limited cell‐membrane permeability. This impacts experimental design as cells with intact membranes, including caspase‐1 knockout cells, are not appropriate controls for cells with inflammasome‐induced gasdermin D membrane pores.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.202350515