Effects of microplastics on photosynthesized C allocation in a rice-soil system and its utilization by soil microbial groups

The effect of microplastics (MPs) on the allocation of rice photosynthetic carbon (C) in paddy systems and its utilization by soil microorganisms remain unclear. In this study, 13C-CO2 pulse labeling was used to quantify the input and allocation of photosynthetic C in a rice–soil system under MPs amendment. Rice was pulse-labeled at tillering growth stage under 0.01% and 1% w/w polyethylene (PE) and polyvinyl chloride (PVC) MP amendments. Plants and soils were sampled 24 h after pulse labeling. Photosynthesized C in roots in MP treatments was 30–54% lower than that in no-MP treatments. The 13C in soil organic C (SOC) in PVC-MP-amended bulk soil was 4.3–4.7 times higher than that in no-MP treatments. PVC and high-dose PE increased the photosynthetic C in microbial biomass C in the rhizosphere soil. MPs altered the allocation of photosynthetic C to microbial phospholipid fatty acid (PLFA) groups. High-dose PVC increased the 13C gram-positive PLFAs. Low-dose PE and high-dose PVC enhanced 13C in fungal PLFAs in bulk soil (including arbuscular mycorrhizal fungi (AMF) and Zygomycota) by 175% and 197%, respectively. The results highlight that MPs alter plant C input and microbial utilization of rhizodeposits, thereby affecting the C cycle in paddy ecosystems. [Display omitted] •Microplastics (MPs) reduce the belowground allocation of rice photosynthetic C.•MPs promote the microbial utilization of rice rhizodeposits in rhizosphere soil.•MP alter the use of rice rhizodeposits by microbial groups.•Low PE and high PVC contents enhance utilization of rice rhizodeposits by fungi.•PVC MP promote the incorporation of rice photosynthetic C in SOC.