Ytterbium 10-carboxyperylene-3,4,9-tricarboxylates for targeted NIR luminescent bioimaging

Two new ytterbium coordination compounds Yb(HPTC)(H 2 O) 2 ( Yb1 ) and Yb(HPTC)(Phen) ( Yb2 ) were obtained using 10-carboxyperylene-3,4,9-tricarboxylate ion (HPTC 3− ) as a sensitizer. Both coordination compounds exhibited intense NIR-II luminescence upon excitation in the visible range and formed stable suspensions with nanoparticles of 50-70 nm in size in an aqueous solution of sodium alginate. Both complexes demonstrated non-toxicity up to at least 25 mg L −1 in two cell cultures: cancer cells MCF7 and embryonic cells HEK293T - making them suitable for bioimaging. For both complexes, the accumulation in cells was directly measured and it was shown that the accumulation of Yb2 was the same for both cell types (0.51-0.52 πg per cell), while Yb1 demonstrated selective accumulation in cancer cells (0.04 πg per cell for HEK293T and 7.00 πg per cell for MCF7). Thus, Yb1 can also be proposed as a selective vis-excited NIR emitting bioprobe. Two new VIS-excited NIR-emitting ytterbium complexes Yb(HPTC)(H 2 O) 2 ( Yb1 ) and Yb(HPTC)(Phen) ( Yb2 ) were obtained. Both compounds demonstrated low toxicity for healthy and cancer cells, moreover Yb1 demonstrated selective accumulation in cancer cells.