Nitrogen and nitrous oxides emission characteristics of anoxic/oxic wastewater treatment process under different oxygen regulation strategies

Nitrous oxide (N2O) and nitrogen oxides (NOx) (i.e., nitric oxide (NO) and nitrogen dioxide (NO2)), which could be produced in wastewater treatment process and result in greenhouse effect and atmospheric pollution, respectively, have been studied limitedly in their emission characteristics and transformation mechanisms. In this study, intelligent oxygen regulation was applied in anoxic/oxic wastewater treatment process (I-A/O), and its effects on regulating NOx and N2O transformations were extensively explored by comparing it with conventional A/O process (C-A/O). Results showed that the average emission amounts of N2O and NOx in I-A/O were 7.45 ± 0.66 mg and 1.88 ± 0.10 mg, respectively. Satisfactory reduction of N2O by 29.28 %–45.08 % was achieved in I-A/O compared to that of C-A/O, but together with increased NOx emission by 83.19 %–120.57 %. Pearson correlation and transcriptional analysis suggested that NO2−-N reduction in the anoxic phase dominated N2O production, while no significant N2O production in the oxic phase was found. Hence, the reduced N2O production in I-A/O was mainly attributed to its efficient denitrification process. On the other hand, both the anoxic and oxic phases played important roles in NO production. More importantly, sufficient oxygen in I-A/O promoted the ammonia oxidation process, resulting in higher NO emission in I-A/O in the oxic phase. The imbalance in NO and N2O emissions was then amplified by the NOR enzyme, which mediates the conversion of NO to N2O in both the anoxic and oxic phases. Besides, carbon emission reduction by 31.32 %–36.50 % was obtained in I-A/O due to aeration consumption savings and greenhouse gas emissions reduction compared to C-A/O. Overall, intelligent oxygen regulation optimized the nitrogen transformation and achieved carbon emission reduction in A/O process, but special attention should be paid to the associated risk caused by increased NO emissions. [Display omitted] •A non-linear positive correlation between NO and N2O emissions was revealed.•Higher NO emission was obtained in I-A/O due to promoted ammonia oxidation.•Reduced N2O production in the anoxic phase led to lower N2O emission in I-A/O.•NOR enzyme amplified the imbalance in NO and N2O emissions.•Carbon emission was reduced by 31.32 %–36.50 % in I-A/O.