Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer
Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the nanoparticles from a bulk solution to a solid surface determine the limit of detection (LOD) and the accuracy o...
Gespeichert in:
| Veröffentlicht in: | Analytical chemistry (Washington) 2024-02, Vol.96 (7), p.3054-3061 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3061 |
|---|---|
| container_issue | 7 |
| container_start_page | 3054 |
| container_title | Analytical chemistry (Washington) |
| container_volume | 96 |
| creator | Zhang, Qingquan Chai, Wenwen Pan, Xiaoyan Gai, Hongwei |
| description | Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the nanoparticles from a bulk solution to a solid surface determine the limit of detection (LOD) and the accuracy of DIAs. Previous methods suffer from issues like low efficiency, nonuniform distribution, and particle aggregation. Here, we present a novel technique named synergistic sedimentation of Brownian motion suppression and dehydration transfer (SynSed) for nanoparticles using water-soluble polymers. The efficiency of transferring quantum dots (QDs) was increased from 10.7 to 91.4%, and the variation in QD distribution was restricted to 8.8%. By incorporating SynSed into DIAs, we achieved a remarkable reduction in the LOD (down to 3.9 aM) for carcinoembryonic antigen and expanded the dynamic range to cover 3 orders of magnitude in concentration, ranging from 0.01 to 10 fM. DIAs enhanced with SynSed possess ultrahigh sensitivity, advanced accuracy, and specificity, offering a great premise in early disease diagnostics, risk stratification, and treatment response monitoring. |
| doi_str_mv | 10.1021/acs.analchem.3c05066 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2923911253</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2923911253</sourcerecordid><originalsourceid>FETCH-LOGICAL-a325t-989d627a9c744994f2bab67e5b32528e7b7a70f6eb90fbf1c93f9733550915f33</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi0EokPhDRCyxIZNhmM7Ny-HlkKlQSymrCPHOe64SuxgO6A8Ca9L5tIuWLDykc_3_5b8EfKWwZoBZx-VjmvlVK_3OKyFhgLK8hlZsYJDVtY1f05WACAyXgFckFcxPgAwBqx8SS5ELbjkMl-RP5th7K2xWiXrXXYTEOm1vbdJ9fR2GCbnVYxqpp3_7WjyNO2RblLyg-9VoFv8hT1tZ7qbHYZ7G5PVdIedHdClYyP1hn4KS9gqR7_549VuGseAMR5m5Tp6jfu5Cyf8LigXDYbX5IVRfcQ35_OS_Lj5fHf1Ndt-_3J7tdlmSvAiZbKWXckrJXWV51LmhreqLSss2mXNa6zaSlVgSmwlmNYwLYWRlRBFAZIVRohL8uHUOwb_c8KYmsFGjX2vHPopNss3CckYLw7o-3_QBz-FxcCRklIKWecLlZ8oHXyMAU0zBjuoMDcMmoO4ZhHXPIprzuKW2Ltz-dQO2D2FHk0tAJyAQ_zp4f92_gWJjKoP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2929993984</pqid></control><display><type>article</type><title>Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer</title><source>ACS Publications</source><creator>Zhang, Qingquan ; Chai, Wenwen ; Pan, Xiaoyan ; Gai, Hongwei</creator><creatorcontrib>Zhang, Qingquan ; Chai, Wenwen ; Pan, Xiaoyan ; Gai, Hongwei</creatorcontrib><description>Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the nanoparticles from a bulk solution to a solid surface determine the limit of detection (LOD) and the accuracy of DIAs. Previous methods suffer from issues like low efficiency, nonuniform distribution, and particle aggregation. Here, we present a novel technique named synergistic sedimentation of Brownian motion suppression and dehydration transfer (SynSed) for nanoparticles using water-soluble polymers. The efficiency of transferring quantum dots (QDs) was increased from 10.7 to 91.4%, and the variation in QD distribution was restricted to 8.8%. By incorporating SynSed into DIAs, we achieved a remarkable reduction in the LOD (down to 3.9 aM) for carcinoembryonic antigen and expanded the dynamic range to cover 3 orders of magnitude in concentration, ranging from 0.01 to 10 fM. DIAs enhanced with SynSed possess ultrahigh sensitivity, advanced accuracy, and specificity, offering a great premise in early disease diagnostics, risk stratification, and treatment response monitoring.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.3c05066</identifier><identifier>PMID: 38329294</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Amplification ; Brownian motion ; Carcinoembryonic antigen ; Dehydration ; Efficiency ; Immunoassay ; Immunoassays ; Nanoparticles ; Polymers ; Quantum dots ; Sedimentation ; Sedimentation & deposition ; Solid surfaces ; Water soluble polymers</subject><ispartof>Analytical chemistry (Washington), 2024-02, Vol.96 (7), p.3054-3061</ispartof><rights>2024 American Chemical Society</rights><rights>Copyright American Chemical Society Feb 20, 2024</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a325t-989d627a9c744994f2bab67e5b32528e7b7a70f6eb90fbf1c93f9733550915f33</cites><orcidid>0000-0002-1069-9543</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.3c05066$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.3c05066$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38329294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Qingquan</creatorcontrib><creatorcontrib>Chai, Wenwen</creatorcontrib><creatorcontrib>Pan, Xiaoyan</creatorcontrib><creatorcontrib>Gai, Hongwei</creatorcontrib><title>Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the nanoparticles from a bulk solution to a solid surface determine the limit of detection (LOD) and the accuracy of DIAs. Previous methods suffer from issues like low efficiency, nonuniform distribution, and particle aggregation. Here, we present a novel technique named synergistic sedimentation of Brownian motion suppression and dehydration transfer (SynSed) for nanoparticles using water-soluble polymers. The efficiency of transferring quantum dots (QDs) was increased from 10.7 to 91.4%, and the variation in QD distribution was restricted to 8.8%. By incorporating SynSed into DIAs, we achieved a remarkable reduction in the LOD (down to 3.9 aM) for carcinoembryonic antigen and expanded the dynamic range to cover 3 orders of magnitude in concentration, ranging from 0.01 to 10 fM. DIAs enhanced with SynSed possess ultrahigh sensitivity, advanced accuracy, and specificity, offering a great premise in early disease diagnostics, risk stratification, and treatment response monitoring.</description><subject>Amplification</subject><subject>Brownian motion</subject><subject>Carcinoembryonic antigen</subject><subject>Dehydration</subject><subject>Efficiency</subject><subject>Immunoassay</subject><subject>Immunoassays</subject><subject>Nanoparticles</subject><subject>Polymers</subject><subject>Quantum dots</subject><subject>Sedimentation</subject><subject>Sedimentation & deposition</subject><subject>Solid surfaces</subject><subject>Water soluble polymers</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kctu1DAUhi0EokPhDRCyxIZNhmM7Ny-HlkKlQSymrCPHOe64SuxgO6A8Ca9L5tIuWLDykc_3_5b8EfKWwZoBZx-VjmvlVK_3OKyFhgLK8hlZsYJDVtY1f05WACAyXgFckFcxPgAwBqx8SS5ELbjkMl-RP5th7K2xWiXrXXYTEOm1vbdJ9fR2GCbnVYxqpp3_7WjyNO2RblLyg-9VoFv8hT1tZ7qbHYZ7G5PVdIedHdClYyP1hn4KS9gqR7_549VuGseAMR5m5Tp6jfu5Cyf8LigXDYbX5IVRfcQ35_OS_Lj5fHf1Ndt-_3J7tdlmSvAiZbKWXckrJXWV51LmhreqLSss2mXNa6zaSlVgSmwlmNYwLYWRlRBFAZIVRohL8uHUOwb_c8KYmsFGjX2vHPopNss3CckYLw7o-3_QBz-FxcCRklIKWecLlZ8oHXyMAU0zBjuoMDcMmoO4ZhHXPIprzuKW2Ltz-dQO2D2FHk0tAJyAQ_zp4f92_gWJjKoP</recordid><startdate>20240208</startdate><enddate>20240208</enddate><creator>Zhang, Qingquan</creator><creator>Chai, Wenwen</creator><creator>Pan, Xiaoyan</creator><creator>Gai, Hongwei</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1069-9543</orcidid></search><sort><creationdate>20240208</creationdate><title>Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer</title><author>Zhang, Qingquan ; Chai, Wenwen ; Pan, Xiaoyan ; Gai, Hongwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a325t-989d627a9c744994f2bab67e5b32528e7b7a70f6eb90fbf1c93f9733550915f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Amplification</topic><topic>Brownian motion</topic><topic>Carcinoembryonic antigen</topic><topic>Dehydration</topic><topic>Efficiency</topic><topic>Immunoassay</topic><topic>Immunoassays</topic><topic>Nanoparticles</topic><topic>Polymers</topic><topic>Quantum dots</topic><topic>Sedimentation</topic><topic>Sedimentation & deposition</topic><topic>Solid surfaces</topic><topic>Water soluble polymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Qingquan</creatorcontrib><creatorcontrib>Chai, Wenwen</creatorcontrib><creatorcontrib>Pan, Xiaoyan</creatorcontrib><creatorcontrib>Gai, Hongwei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Qingquan</au><au>Chai, Wenwen</au><au>Pan, Xiaoyan</au><au>Gai, Hongwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2024-02-08</date><risdate>2024</risdate><volume>96</volume><issue>7</issue><spage>3054</spage><epage>3061</epage><pages>3054-3061</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Amplification-free digital immunoassays (DIAs) typically utilize optical nanoparticles to enhance single immunocomplex molecule detection. The efficiency and uniformity of transferring the nanoparticles from a bulk solution to a solid surface determine the limit of detection (LOD) and the accuracy of DIAs. Previous methods suffer from issues like low efficiency, nonuniform distribution, and particle aggregation. Here, we present a novel technique named synergistic sedimentation of Brownian motion suppression and dehydration transfer (SynSed) for nanoparticles using water-soluble polymers. The efficiency of transferring quantum dots (QDs) was increased from 10.7 to 91.4%, and the variation in QD distribution was restricted to 8.8%. By incorporating SynSed into DIAs, we achieved a remarkable reduction in the LOD (down to 3.9 aM) for carcinoembryonic antigen and expanded the dynamic range to cover 3 orders of magnitude in concentration, ranging from 0.01 to 10 fM. DIAs enhanced with SynSed possess ultrahigh sensitivity, advanced accuracy, and specificity, offering a great premise in early disease diagnostics, risk stratification, and treatment response monitoring.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>38329294</pmid><doi>10.1021/acs.analchem.3c05066</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1069-9543</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2700 |
| ispartof | Analytical chemistry (Washington), 2024-02, Vol.96 (7), p.3054-3061 |
| issn | 0003-2700 1520-6882 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2923911253 |
| source | ACS Publications |
| subjects | Amplification Brownian motion Carcinoembryonic antigen Dehydration Efficiency Immunoassay Immunoassays Nanoparticles Polymers Quantum dots Sedimentation Sedimentation & deposition Solid surfaces Water soluble polymers |
| title | Amplification-Free Digital Immunoassay down to the Attomolar Level by Synergistic Sedimentation of Brownian Motion Suppression and Dehydration Transfer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T16%3A56%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amplification-Free%20Digital%20Immunoassay%20down%20to%20the%20Attomolar%20Level%20by%20Synergistic%20Sedimentation%20of%20Brownian%20Motion%20Suppression%20and%20Dehydration%20Transfer&rft.jtitle=Analytical%20chemistry%20(Washington)&rft.au=Zhang,%20Qingquan&rft.date=2024-02-08&rft.volume=96&rft.issue=7&rft.spage=3054&rft.epage=3061&rft.pages=3054-3061&rft.issn=0003-2700&rft.eissn=1520-6882&rft_id=info:doi/10.1021/acs.analchem.3c05066&rft_dat=%3Cproquest_cross%3E2923911253%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2929993984&rft_id=info:pmid/38329294&rfr_iscdi=true |