High‐speed centrifugation rather than Lipoclear reagent can be used for removing the interference of lipemia on serological tests of infectious diseases: AIDS, hepatitis B, hepatitis C, and syphilis by chemiluminescent microparticle immunoassay

The aim of this study was to investigate the interference of lipemia on measurement of HBsAg, anti‐HBs, HBeAg, anti‐HBe, anti‐HBc, anti‐HCV, HIV Ag/Ab, and anti‐TP in serum by chemiluminescent microparticle immunoassay (CMIA) and compare lipemia removing performance between high‐speed centrifugation and Lipoclear reagent. Mixed native serum samples (NSs) and hyperlipemia serum samples (HLS) were prepared for the investigated parameters. The levels of these parameters in NS and HLS were determined by CMIA on an Abbott ARCHITECT i2000SR immunoassay analyzer. HBsAg, anti‐HBs, and anti‐TP were affected with relative bias >12.5% (acceptable limit) when the level of triacylglycerol (TG) was higher than 27.12 mmol/L in HLS. Clinically unacceptable bias were observed for HBeAg and anti‐HBe in HLS with TG higher than 40.52 mmol/L. However, anti‐HCV and HIV Ag/Ab were not interfered in severe lipemia with TG