Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?
Objective To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions. Study Design The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non‐syndromic odontoge...
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| Veröffentlicht in: | Oral diseases 2024-10, Vol.30 (7), p.4538-4546 |
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| creator | Gonçalo, Rani Iani Costa Sousa, Julliany Taverny Costa, Carla Samily de Oliveira Mafra, Rodrigo Porpino Santos, Janaina Lessa de Moraes dos Silva, Leorik Pereira Queiroz, Lélia Maria Guedes |
| description | Objective
To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.
Study Design
The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non‐syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki‐67, and Bcl‐2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).
Results
The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki‐67 in unicystic ameloblastomas (p |
| doi_str_mv | 10.1111/odi.14882 |
| format | Article |
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To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.
Study Design
The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non‐syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki‐67, and Bcl‐2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).
Results
The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki‐67 in unicystic ameloblastomas (p < 0.05). However, there were no statistically significant correlations between YAP and Bcl‐2 immunoexpression in the groups studied.
Conclusion
YAP may influence epithelial cell proliferation in odontogenic cysts and tumors, suggesting its possible participation in the progression of the odontogenic lesions studied.</description><identifier>ISSN: 1354-523X</identifier><identifier>ISSN: 1601-0825</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14882</identifier><identifier>PMID: 38297810</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adaptor Proteins, Signal Transducing - metabolism ; Ameloblastoma - metabolism ; Ameloblastoma - pathology ; Apoptosis ; Cell growth ; Cell Proliferation ; Cyclin D1 ; Cyclin D1 - metabolism ; Cysts ; Dental Sac - metabolism ; Dental Sac - pathology ; Dentigerous Cyst - metabolism ; Dentigerous Cyst - pathology ; Epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - pathology ; Follicles ; Humans ; Immunohistochemistry ; Ki-67 Antigen - analysis ; Ki-67 Antigen - metabolism ; Lesions ; odontogenic cysts ; Odontogenic Cysts - metabolism ; Odontogenic Cysts - pathology ; odontogenic tumors ; Odontogenic Tumors - metabolism ; Odontogenic Tumors - pathology ; Proto-Oncogene Proteins c-bcl-2 - analysis ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Statistical analysis ; Transcription Factors - analysis ; YAP-Signaling Proteins ; Yes-associated protein</subject><ispartof>Oral diseases, 2024-10, Vol.30 (7), p.4538-4546</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-2bfe23f7cac478b8dbd463e3fdbe688b09dedff5cb4ffddc475361e6881197b13</citedby><cites>FETCH-LOGICAL-c3532-2bfe23f7cac478b8dbd463e3fdbe688b09dedff5cb4ffddc475361e6881197b13</cites><orcidid>0000-0001-7600-4321 ; 0000-0003-4390-1434 ; 0000-0002-1392-4259 ; 0000-0002-6762-135X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14882$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14882$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38297810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonçalo, Rani Iani Costa</creatorcontrib><creatorcontrib>Sousa, Julliany Taverny</creatorcontrib><creatorcontrib>Costa, Carla Samily de Oliveira</creatorcontrib><creatorcontrib>Mafra, Rodrigo Porpino</creatorcontrib><creatorcontrib>Santos, Janaina Lessa de Moraes dos</creatorcontrib><creatorcontrib>Silva, Leorik Pereira</creatorcontrib><creatorcontrib>Queiroz, Lélia Maria Guedes</creatorcontrib><title>Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objective
To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.
Study Design
The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non‐syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki‐67, and Bcl‐2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).
Results
The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki‐67 in unicystic ameloblastomas (p < 0.05). However, there were no statistically significant correlations between YAP and Bcl‐2 immunoexpression in the groups studied.
Conclusion
YAP may influence epithelial cell proliferation in odontogenic cysts and tumors, suggesting its possible participation in the progression of the odontogenic lesions studied.</description><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Ameloblastoma - metabolism</subject><subject>Ameloblastoma - pathology</subject><subject>Apoptosis</subject><subject>Cell growth</subject><subject>Cell Proliferation</subject><subject>Cyclin D1</subject><subject>Cyclin D1 - metabolism</subject><subject>Cysts</subject><subject>Dental Sac - metabolism</subject><subject>Dental Sac - pathology</subject><subject>Dentigerous Cyst - metabolism</subject><subject>Dentigerous Cyst - pathology</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - pathology</subject><subject>Follicles</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Ki-67 Antigen - analysis</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Lesions</subject><subject>odontogenic cysts</subject><subject>Odontogenic Cysts - metabolism</subject><subject>Odontogenic Cysts - pathology</subject><subject>odontogenic tumors</subject><subject>Odontogenic Tumors - metabolism</subject><subject>Odontogenic Tumors - pathology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - analysis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Statistical analysis</subject><subject>Transcription Factors - analysis</subject><subject>YAP-Signaling Proteins</subject><subject>Yes-associated protein</subject><issn>1354-523X</issn><issn>1601-0825</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LJDEQhoOs-H3wD0hgL-uhNR-d7vRpkdHdFQb0oKAHCZ1Oxc2QSXo73cj8ezM76kEwlwpVT14qD0LHlJzRfM6jcWe0lJJtoT1aEVoQycS3fOeiLATjD7toP6UFIbRuONtBu1yyppaU7KGnywgJP17cYhesnyB0gDvwHvdD9M7C0I4uBtwGg9s-9mNMLmUUawjuOWDo3fgXvGs9jiaGMT7nfoc9pPwq_TxE27b1CY7e6gG6_3V1N_tTzG9-X88u5kXHBWcF0xYYt3XXdmUttTTalBUHbo2GSkpNGgPGWtHp0lpjMiR4RdcjSptaU36Afmxy89b_JkijWrq0_kYbIE5JsYZlT0LWJKPfP6GLOA0hb6c4pbwSoixFpk43VDfElAawqh_csh1WihK1dq6yc_XfeWZP3hInvQTzQb5LzsD5BnhxHlZfJ6mby-tN5Ct71Iwk</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Gonçalo, Rani Iani Costa</creator><creator>Sousa, Julliany Taverny</creator><creator>Costa, Carla Samily de Oliveira</creator><creator>Mafra, Rodrigo Porpino</creator><creator>Santos, Janaina Lessa de Moraes dos</creator><creator>Silva, Leorik Pereira</creator><creator>Queiroz, Lélia Maria Guedes</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7600-4321</orcidid><orcidid>https://orcid.org/0000-0003-4390-1434</orcidid><orcidid>https://orcid.org/0000-0002-1392-4259</orcidid><orcidid>https://orcid.org/0000-0002-6762-135X</orcidid></search><sort><creationdate>202410</creationdate><title>Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?</title><author>Gonçalo, Rani Iani Costa ; Sousa, Julliany Taverny ; Costa, Carla Samily de Oliveira ; Mafra, Rodrigo Porpino ; Santos, Janaina Lessa de Moraes dos ; Silva, Leorik Pereira ; Queiroz, Lélia Maria Guedes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-2bfe23f7cac478b8dbd463e3fdbe688b09dedff5cb4ffddc475361e6881197b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Ameloblastoma - metabolism</topic><topic>Ameloblastoma - pathology</topic><topic>Apoptosis</topic><topic>Cell growth</topic><topic>Cell Proliferation</topic><topic>Cyclin D1</topic><topic>Cyclin D1 - metabolism</topic><topic>Cysts</topic><topic>Dental Sac - metabolism</topic><topic>Dental Sac - pathology</topic><topic>Dentigerous Cyst - metabolism</topic><topic>Dentigerous Cyst - pathology</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - pathology</topic><topic>Follicles</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Ki-67 Antigen - analysis</topic><topic>Ki-67 Antigen - metabolism</topic><topic>Lesions</topic><topic>odontogenic cysts</topic><topic>Odontogenic Cysts - metabolism</topic><topic>Odontogenic Cysts - pathology</topic><topic>odontogenic tumors</topic><topic>Odontogenic Tumors - metabolism</topic><topic>Odontogenic Tumors - pathology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - analysis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Statistical analysis</topic><topic>Transcription Factors - analysis</topic><topic>YAP-Signaling Proteins</topic><topic>Yes-associated protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonçalo, Rani Iani Costa</creatorcontrib><creatorcontrib>Sousa, Julliany Taverny</creatorcontrib><creatorcontrib>Costa, Carla Samily de Oliveira</creatorcontrib><creatorcontrib>Mafra, Rodrigo Porpino</creatorcontrib><creatorcontrib>Santos, Janaina Lessa de Moraes dos</creatorcontrib><creatorcontrib>Silva, Leorik Pereira</creatorcontrib><creatorcontrib>Queiroz, Lélia Maria Guedes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonçalo, Rani Iani Costa</au><au>Sousa, Julliany Taverny</au><au>Costa, Carla Samily de Oliveira</au><au>Mafra, Rodrigo Porpino</au><au>Santos, Janaina Lessa de Moraes dos</au><au>Silva, Leorik Pereira</au><au>Queiroz, Lélia Maria Guedes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2024-10</date><risdate>2024</risdate><volume>30</volume><issue>7</issue><spage>4538</spage><epage>4546</epage><pages>4538-4546</pages><issn>1354-523X</issn><issn>1601-0825</issn><eissn>1601-0825</eissn><abstract>Objective
To analyze the immunohistochemical expression of YAP and its correlation with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions.
Study Design
The sample consisted of 95 cases of odontogenic lesions (25 dentigerous cysts, 30 non‐syndromic odontogenic keratocysts, 30 conventional ameloblastomas, and 10 unicystic ameloblastomas) and 10 dental follicles used as normal odontogenic tissue. The histological sections were submitted to immunohistochemistry with YAP, cyclin D1, Ki‐67, and Bcl‐2 antibodies. Immunoexpression was analyzed qualitatively and quantitatively using an adapted method. The collected data were analyzed descriptively and statistically (p ≤ 0.05).
Results
The highest YAP expression was observed in odontogenic keratocysts, followed by unicystic ameloblastomas and conventional ameloblastomas, which exhibited moderate immunoreactivity predominantly in peripheral cells. Furthermore, significant differences in YAP immunoexpression were observed between the groups analyzed, with significant positive correlations between YAP and cyclin D1 in dentigerous cysts and unicystic ameloblastomas and between YAP and Ki‐67 in unicystic ameloblastomas (p < 0.05). However, there were no statistically significant correlations between YAP and Bcl‐2 immunoexpression in the groups studied.
Conclusion
YAP may influence epithelial cell proliferation in odontogenic cysts and tumors, suggesting its possible participation in the progression of the odontogenic lesions studied.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38297810</pmid><doi>10.1111/odi.14882</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7600-4321</orcidid><orcidid>https://orcid.org/0000-0003-4390-1434</orcidid><orcidid>https://orcid.org/0000-0002-1392-4259</orcidid><orcidid>https://orcid.org/0000-0002-6762-135X</orcidid></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - metabolism Ameloblastoma - metabolism Ameloblastoma - pathology Apoptosis Cell growth Cell Proliferation Cyclin D1 Cyclin D1 - metabolism Cysts Dental Sac - metabolism Dental Sac - pathology Dentigerous Cyst - metabolism Dentigerous Cyst - pathology Epithelial cells Epithelial Cells - metabolism Epithelial Cells - pathology Follicles Humans Immunohistochemistry Ki-67 Antigen - analysis Ki-67 Antigen - metabolism Lesions odontogenic cysts Odontogenic Cysts - metabolism Odontogenic Cysts - pathology odontogenic tumors Odontogenic Tumors - metabolism Odontogenic Tumors - pathology Proto-Oncogene Proteins c-bcl-2 - analysis Proto-Oncogene Proteins c-bcl-2 - metabolism Statistical analysis Transcription Factors - analysis YAP-Signaling Proteins Yes-associated protein |
| title | Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions? |
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