Self-assembly of polyaniline-grafted chitosan/glucose oxidase nanolayered films for electrochemical biosensor applications

In the present study, chitosan film via graft copolymerization with electroactive PAN was investigated. The modified polymer was subsequently functionalized via layer-by-layer electrostatic self-assembly immobilization of glucose oxidase (GOD). Previous work revealed that surface graft copolymerization of chitosan film with electroactive PAN readily increased the conductivity while remained the hydrophilicity and the biocompatibility. The multilayer membrane is expected to have a rapid response and a better sensitivity simultaneous with a higher response current as the layer number increases.