Knockdown of the ABCG23 Gene Disrupts the Development and Lipid Accumulation of Panonychus citri (Acari/Tetranychidae)

is a worldwide citrus pest that is currently controlled through the use of insecticides. However, alternative strategies are required to manage . Recent studies suggest that the ATP-binding cassette (ABC) transporter G subfamily plays a crucial role in transporting cuticular lipids, which are essential for the insect's barrier function against microbial penetration. Therefore, investigating the potential of the ABC transporter G subfamily as a control measure for could be a promising approach. Based on the genome database, the gene was cloned, and the transcriptional response of for the different developmental stages of and under spirobudiclofen stress was investigated. Our results showed that the expression level of was significantly lower in adult females exposed to treatment compared to the control and was higher in females than males. The knockdown of using RNAi led to a decrease in the survival rate, fecundity, and TG contents of . Additionally, a lethal phenotype was characterized by body wrinkling and darkening. These results indicate that may be involved in cuticular lipid transportation and have adverse effects on the development and reproduction of , providing insight into the discovery of new targets for pest management based on the insect cuticle's penetration barrier function.