Integrating ecology, physiology and transcriptomics reveals the response of Zostera marina to rusting of iron transplantation frame

Frame Transplantation System (FTS) is considered an efficient method for seagrass restoration, but the effect of the rusting of iron frame on seagrass restoration remains unclear. We transplanted Zostera marina plants using iron FTS treated with fluorocarbon paint (painted treatment, PT) and traditional unpainted iron FTS (unpainted treatment, UT) under controlled mesocosm conditions for 24 days. Our results showed that the survival rate of Z. marina under the UT was significantly 31.2 % lower than that of the plants under the PT. Soluble sugar content in Z. marina rhizomes under the UT was significantly 2.19 times higher than that of the plants under the PT. Transcriptome analysis revealed differentially expressed genes (DEGs) involved in photosynthesis, metabolism and signal transduction functions. The results provide valuable data that could prove helpful in the development of efficient restoration techniques for Z. marina beds. •The rusting of iron frame had a significantly negative impact on Z. marina survival and growth.•Z. marina plants resisted stressful environments by increasing photosynthetic pigment and soluble sugar contents.•The rusting of iron frame significantly affected the gene expression in Z. marina plants.