Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells
Ratiometric imaging of tumor‐related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non‐specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for...
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| Veröffentlicht in: | Advanced healthcare materials 2024-03, Vol.13 (8), p.e2303074-n/a |
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| creator | Zhang, Fuqiang Yang, Nan Zhou, Fu Qiao, Ruonan Wan, Yifei Liu, Rong Yang, Suwan Gu, Mingzheng Xu, Huae Dong, Xiaochen Wang, Guangfeng |
| description | Ratiometric imaging of tumor‐related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non‐specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and Ratiometric (STAR) imaging of TK1 mRNA inside living cells with enhanced reliability and accuracy. By virtue of the synthesized CuO/MnO2 nanosheets, orthogonally regulated self‐powered DNAzyme circuits are operated precisely in living cells, sequentially activating two‐layered DNAzyme cleavage reactions to achieve the two ratiometric signal readouts successively for reliable monitoring of low‐abundance mRNA in living cells. It is found that the ratiometric signals can only be derived from mRNA over‐expressed tumor cells, also irrespective of probes’ delivery concentration. The presented approach could provide new insight into orthogonally regulated ratiometric systems for reliable imaging of specific biomarkers in living cells, benefiting disease precision diagnostics.
Ratiometric and amplified fluorescent monitoring of TK1 mRNA in living tumor cells with high reliability is achieved by orthogonally regulated self‐powered DNAzymes cascades. |
| doi_str_mv | 10.1002/adhm.202303074 |
| format | Article |
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Ratiometric and amplified fluorescent monitoring of TK1 mRNA in living tumor cells with high reliability is achieved by orthogonally regulated self‐powered DNAzymes cascades.</description><identifier>ISSN: 2192-2640</identifier><identifier>ISSN: 2192-2659</identifier><identifier>EISSN: 2192-2659</identifier><identifier>DOI: 10.1002/adhm.202303074</identifier><identifier>PMID: 38197479</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Biomarkers ; Cell activation ; Cells (biology) ; Circuits ; CuO/MnO2 ; Deoxyribozymes ; DNAzyme cascade ; Imaging ; Manganese dioxide ; Monitoring ; mRNA ; orthogonal activation ; ratiometric imaging ; self‐powered ; Tumor cells ; Tumors</subject><ispartof>Advanced healthcare materials, 2024-03, Vol.13 (8), p.e2303074-n/a</ispartof><rights>2024 Wiley‐VCH GmbH</rights><rights>2024 Wiley-VCH GmbH.</rights><rights>2024 Wiley‐VCH GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3734-88d7907628c1829c8de84f7dffcf2944973680ee91a43c7b43841684093a2ff43</citedby><cites>FETCH-LOGICAL-c3734-88d7907628c1829c8de84f7dffcf2944973680ee91a43c7b43841684093a2ff43</cites><orcidid>0000-0001-7327-8060</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fadhm.202303074$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fadhm.202303074$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38197479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Fuqiang</creatorcontrib><creatorcontrib>Yang, Nan</creatorcontrib><creatorcontrib>Zhou, Fu</creatorcontrib><creatorcontrib>Qiao, Ruonan</creatorcontrib><creatorcontrib>Wan, Yifei</creatorcontrib><creatorcontrib>Liu, Rong</creatorcontrib><creatorcontrib>Yang, Suwan</creatorcontrib><creatorcontrib>Gu, Mingzheng</creatorcontrib><creatorcontrib>Xu, Huae</creatorcontrib><creatorcontrib>Dong, Xiaochen</creatorcontrib><creatorcontrib>Wang, Guangfeng</creatorcontrib><title>Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells</title><title>Advanced healthcare materials</title><addtitle>Adv Healthc Mater</addtitle><description>Ratiometric imaging of tumor‐related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non‐specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and Ratiometric (STAR) imaging of TK1 mRNA inside living cells with enhanced reliability and accuracy. By virtue of the synthesized CuO/MnO2 nanosheets, orthogonally regulated self‐powered DNAzyme circuits are operated precisely in living cells, sequentially activating two‐layered DNAzyme cleavage reactions to achieve the two ratiometric signal readouts successively for reliable monitoring of low‐abundance mRNA in living cells. It is found that the ratiometric signals can only be derived from mRNA over‐expressed tumor cells, also irrespective of probes’ delivery concentration. The presented approach could provide new insight into orthogonally regulated ratiometric systems for reliable imaging of specific biomarkers in living cells, benefiting disease precision diagnostics.
Ratiometric and amplified fluorescent monitoring of TK1 mRNA in living tumor cells with high reliability is achieved by orthogonally regulated self‐powered DNAzymes cascades.</description><subject>Biomarkers</subject><subject>Cell activation</subject><subject>Cells (biology)</subject><subject>Circuits</subject><subject>CuO/MnO2</subject><subject>Deoxyribozymes</subject><subject>DNAzyme cascade</subject><subject>Imaging</subject><subject>Manganese dioxide</subject><subject>Monitoring</subject><subject>mRNA</subject><subject>orthogonal activation</subject><subject>ratiometric imaging</subject><subject>self‐powered</subject><subject>Tumor cells</subject><subject>Tumors</subject><issn>2192-2640</issn><issn>2192-2659</issn><issn>2192-2659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1O3DAUha2qVUHAtsvKUjfdzOC_ie1lNLSl0gAVbdeRx7kGIyemdgKarngEnrFPgqOBqdRNvbmW73ePfM9B6B0lc0oIOzbtdTdnhHHCiRSv0D6jms1YtdCvd3dB9tBRzjeknGpBK0Xfoj2uqJZC6n00XqThOl7F3oSwwd_h1wj94E3AtR38nRl87HF0pRHcn4fHb_EeErT45Lz-vekg46XJ1rSAXUz4EoI36wD4LPZ-iMn3V9Nod3leY9_jlb-bXpYQQj5Eb5wJGY6e6wH6-fnTj-XpbHXx5euyXs0sl1zMlGqlJrJiylLFtFUtKOFk65x1TAuhJa8UAdDUCG7lWnAlyoaCaG6Yc4IfoI9b3dsUy2Z5aDqfbfmB6SGOuWGaclK8oLqgH_5Bb-KYii0TpaoFEcXjQs23lE0x5wSuuU2-M2nTUNJMmTRTJs0ukzLw_ll2XHfQ7vCXBAqgt8C9D7D5j1xTn5ye_RV_AlhNl8U</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Zhang, Fuqiang</creator><creator>Yang, Nan</creator><creator>Zhou, Fu</creator><creator>Qiao, Ruonan</creator><creator>Wan, Yifei</creator><creator>Liu, Rong</creator><creator>Yang, Suwan</creator><creator>Gu, Mingzheng</creator><creator>Xu, Huae</creator><creator>Dong, Xiaochen</creator><creator>Wang, Guangfeng</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T5</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7TO</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7327-8060</orcidid></search><sort><creationdate>20240301</creationdate><title>Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells</title><author>Zhang, Fuqiang ; Yang, Nan ; Zhou, Fu ; Qiao, Ruonan ; Wan, Yifei ; Liu, Rong ; Yang, Suwan ; Gu, Mingzheng ; Xu, Huae ; Dong, Xiaochen ; Wang, Guangfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3734-88d7907628c1829c8de84f7dffcf2944973680ee91a43c7b43841684093a2ff43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomarkers</topic><topic>Cell activation</topic><topic>Cells (biology)</topic><topic>Circuits</topic><topic>CuO/MnO2</topic><topic>Deoxyribozymes</topic><topic>DNAzyme cascade</topic><topic>Imaging</topic><topic>Manganese dioxide</topic><topic>Monitoring</topic><topic>mRNA</topic><topic>orthogonal activation</topic><topic>ratiometric imaging</topic><topic>self‐powered</topic><topic>Tumor cells</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Fuqiang</creatorcontrib><creatorcontrib>Yang, Nan</creatorcontrib><creatorcontrib>Zhou, Fu</creatorcontrib><creatorcontrib>Qiao, Ruonan</creatorcontrib><creatorcontrib>Wan, Yifei</creatorcontrib><creatorcontrib>Liu, Rong</creatorcontrib><creatorcontrib>Yang, Suwan</creatorcontrib><creatorcontrib>Gu, Mingzheng</creatorcontrib><creatorcontrib>Xu, Huae</creatorcontrib><creatorcontrib>Dong, Xiaochen</creatorcontrib><creatorcontrib>Wang, Guangfeng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Immunology Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Advanced healthcare materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Fuqiang</au><au>Yang, Nan</au><au>Zhou, Fu</au><au>Qiao, Ruonan</au><au>Wan, Yifei</au><au>Liu, Rong</au><au>Yang, Suwan</au><au>Gu, Mingzheng</au><au>Xu, Huae</au><au>Dong, Xiaochen</au><au>Wang, Guangfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells</atitle><jtitle>Advanced healthcare materials</jtitle><addtitle>Adv Healthc Mater</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>13</volume><issue>8</issue><spage>e2303074</spage><epage>n/a</epage><pages>e2303074-n/a</pages><issn>2192-2640</issn><issn>2192-2659</issn><eissn>2192-2659</eissn><abstract>Ratiometric imaging of tumor‐related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non‐specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and Ratiometric (STAR) imaging of TK1 mRNA inside living cells with enhanced reliability and accuracy. By virtue of the synthesized CuO/MnO2 nanosheets, orthogonally regulated self‐powered DNAzyme circuits are operated precisely in living cells, sequentially activating two‐layered DNAzyme cleavage reactions to achieve the two ratiometric signal readouts successively for reliable monitoring of low‐abundance mRNA in living cells. It is found that the ratiometric signals can only be derived from mRNA over‐expressed tumor cells, also irrespective of probes’ delivery concentration. The presented approach could provide new insight into orthogonally regulated ratiometric systems for reliable imaging of specific biomarkers in living cells, benefiting disease precision diagnostics.
Ratiometric and amplified fluorescent monitoring of TK1 mRNA in living tumor cells with high reliability is achieved by orthogonally regulated self‐powered DNAzymes cascades.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>38197479</pmid><doi>10.1002/adhm.202303074</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-7327-8060</orcidid></addata></record> |
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| subjects | Biomarkers Cell activation Cells (biology) Circuits CuO/MnO2 Deoxyribozymes DNAzyme cascade Imaging Manganese dioxide Monitoring mRNA orthogonal activation ratiometric imaging self‐powered Tumor cells Tumors |
| title | Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells |
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