Orthogonally Sequential Activation of Self‐Powered DNAzymes Cascade for Reliable Monitoring of mRNA in Living Cells

Ratiometric imaging of tumor‐related mRNA is significant, yet spatiotemporally resolved regulation on the ratiometric signals to avoid non‐specific activation in the living cells remains challenging. Herein, orthogonally sequential activation of concatenated DNAzyme circuits is, first, developed for Spatio Temporally regulated Amplified and Ratiometric (STAR) imaging of TK1 mRNA inside living cells with enhanced reliability and accuracy. By virtue of the synthesized CuO/MnO2 nanosheets, orthogonally regulated self‐powered DNAzyme circuits are operated precisely in living cells, sequentially activating two‐layered DNAzyme cleavage reactions to achieve the two ratiometric signal readouts successively for reliable monitoring of low‐abundance mRNA in living cells. It is found that the ratiometric signals can only be derived from mRNA over‐expressed tumor cells, also irrespective of probes’ delivery concentration. The presented approach could provide new insight into orthogonally regulated ratiometric systems for reliable imaging of specific biomarkers in living cells, benefiting disease precision diagnostics. Ratiometric and amplified fluorescent monitoring of TK1 mRNA in living tumor cells with high reliability is achieved by orthogonally regulated self‐powered DNAzymes cascades.