Comamonas endophytica sp. nov., a novel indole acetic acid producing endophyte isolated from bamboo in China

Two novel indole acetic acid-producing strains, 5MLIR and D4N7, were isolated from in Yongzhou, Hunan province, and in Hangzhou, Zhejiang province, respectively. Based on their sequences, strains 5MLIR and D4N7 were closely related to 16-35-5 (98.4 % sequence similarity), and the results of 92-core gene phylogenetic trees showed that strains 5MLIR and D4N7 formed a phylogenetic lineage within the clade comprising species. The complete genome size of strain 5MLIR was 4.49 Mb including two plasmids, and the DNA G+C content was 66.5 mol%. The draft genome of strain D4N7 was 4.26 Mb with 66.7 mol% G+C content. The average nucleotide identity and digital DNA-DNA hybridization values among strain 5MLIR and species in the genus were all below the species delineation threshold. The colonies of strain 5MLIR and D4N7 were circular with regular margins, convex, pale yellow and 1.0-2.0 mm in diameter when incubated at 30 °C for 3 days. Strains 5MLIR and D4N7 grew optimally at 30 °C, pH 7.0 and 1.0 % NaCl. The respiratory isoprenoid quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Polyphasic analyses indicated that strains 5MLIR and D4N7 could be distinguished from related validly named species and represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5MLIR (=ACCC 62069 =GDMCC 1.2958 =JCM 35331 ).