Biomolecular linear motors confined to move upon micro-patterns on glass

Biomolecular linear motor proteins-kinesin and microtubules-are finely patterned on a conventional, flat glass substrate using a Parylene lift-off process. These patterns are useful for single molecule analysis and biohybrid transportation. A patterning width of 2 mum has been achieved with minimal...

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Veröffentlicht in:	Journal of micromechanics and microengineering 2006-08, Vol.16 (8), p.1550-1554
Hauptverfasser:	Yoshida, Yumi, Yokokawa, Ryuji, Suzuki, Hiroaki, Atsuta, Kyoko, Fujita, Hiroyuki, Takeuchi, Shoji
Format:	Artikel
Sprache:	eng
Schlagworte:	Applied sciences Electronics Exact sciences and technology Instruments, apparatus, components and techniques common to several branches of physics and astronomy Mechanical engineering. Machine design Mechanical instruments, equipment and techniques Microelectronic fabrication (materials and surfaces technology) Micromechanical devices and systems Physics Precision engineering, watch making Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
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Beschreibung
Zusammenfassung:	Biomolecular linear motor proteins-kinesin and microtubules-are finely patterned on a conventional, flat glass substrate using a Parylene lift-off process. These patterns are useful for single molecule analysis and biohybrid transportation. A patterning width of 2 mum has been achieved with minimal non-specific binding of the proteins. A conventional gliding assay was realized using this glass substrate and behaviors of the single proteins were analyzed.
ISSN:	0960-1317 1361-6439
DOI:	10.1088/0960-1317/16/8/015