Tenacibaculum tangerinum sp. nov., isolated from a tidal flat sediment

An orange-coloured bacterium, designated as strain GRR-S3-23 , was isolated from a tidal flat sediment collected from Garorim Bay, Chuncheongbuk-do, Republic of Korea. Cells of GRR-S3-23 were aerobic, Gram-stain-negative, rod-shaped and motile. GRR-S3-23 grew at 18-40 °C (optimum, 30 °C), pH 7.0-9.0 (optimum, pH 7.0) and with 2-4 % NaCl (optimum, 2-3 % w/v). Results of 16S rRNA gene sequence analysis indicated that GRR-S3-23 was closely related to a4 (97.6 %), followed by SMK-4 (97.5 %), MBIC 1140 (97.4 %), TLL-A2 (97.3 %), JO-1 (97.2 %),and YKTF-3 (97.1 %). The average amino acid identity values between GRR-S3-23 and the related strains were 86.8-72.8 %, the average nucleotide identity values were 83.3-74.1 %, and the digital DNA-DNA hybridization values were 27.0-19.6 %. GRR-S3-23 possessed menaquinone-6 (MK-6) as major respiratory quinone and had summed feature 3 (C ω7 and/or C ω6 , 20.6 %) and iso-C G (10.8 %) as major fatty acids (>10.0 %). The polar lipid profiles of GRR-S3-23 contained phosphatidylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid, three unidentified lipids, one unidentified glycolipid and four unidentified phospholipids. The DNA G+C content of GRR-S3-23 was 33.7%. On the basis of the results of the polyphasic analysis involving phylogenetic, phylogenomic, physiological and chemotaxonomic analyses described in this study, GRR-S3-23 is considered to represent a novel species within the genus , for which the name is proposed. The type strain is GRR-S3-23 (=KCTC 102029 =KACC 23271 =JCM 36353 ).