A multifunctional fluorescent sensor for Ag+ and Hg2+ detection in seawater

In order to detect Ag + and Hg 2+ in seawater, we explored a multifunctional fluorescence sensor. A multifunctional Ag + and Hg 2+ sensor was designed by using gold nanoparticles (AuNPs) as quenching agent, PicoGreen dye as fluorescent probe of base pairing double-stranded deoxyribonucleic acid (DNA), and combining the characteristics of Ag + making C base mismatch and Hg 2+ making T base mismatch. Meanwhile, the DNA logic gate was constructed by establishing logic circuit, truth table, and logic formula. The relevant performances of the sensor were investigated. The results revealed that the sensor can detect Ag + in the range of 100 to 700 nM with R 2 = 0.98129, and its detection limit is 16.88 nM (3σ/slope). The detection range of Hg 2+ is 100–900 nM with R 2 = 0.99725, and the detection limit is 5.59 nM (3σ/slope). An AND-AND-NOR-AND molecular logic gate has been successfully designed. With the characteristics of high sensitivity, multifunction, and low cost, the recommended detection method has the potential to be applied to the detection of Ag + and Hg 2+ in seawater.