Superoxide dismutase-like cerium dioxide hollow sphere-based highly specific photoelectrochemical biosensing for ascorbic acid

Conventional N-type semiconductor-based photoelectrochemical (PEC) sensors are difficult to achieve high selectivity for ascorbic acid (AA) detection in real samples because co-existing reducing agents act as hole sacrificial agents like AA to promote the increase of photocurrent. Cerium dioxide (CeO2) is a superoxide dismutase-like nanozyme with the reversible Ce3+/Ce4+ redox pair as well as one of alternative N-type semiconductors. To address the problem of PEC detection selectivity of AA, bifunctional CeO2 is a good choice. Herein, a novel and rational PEC biosensor for AA is constructed based on CeO2 hollow spheres as both AA superoxide dismutase-like nanozyme and the photoelectric beacon, which enable the PEC approach with high selectivity. In this protocol, AA can selectively induce a decrease in the CeO2-based photoanode current, which is significantly different from the conventional N-type semiconductor-based PEC sensor, this unique working mechanism is also proposed. The results show that the CeO2-based photocurrent response decreases linearly with AA concentrations in the ranges of 1 μM–600 μM and 600 μM–3000 μM, with a limit of detection of 0.33 μM. Moreover, the fabricated PEC biosensor has advantages of cost-effectiveness, replicability, and stability. Additionally, the sensor is competent for AA determination in practical settings and has achieved satisfactory results. [Display omitted] •A novel PEC biosensor for AA is constructed based on a unique sensing mechanism.•CeO2 acts as both superoxide dismutase-like nanozyme and photoelectric beacon roles.•This exclusive working mechanism enables the PEC sensor with high specificity.•It has additional strengths of simplicity, low cost, replicability, and stability.•It provides new perspectives for AA assay and extends the application of CeO2.