Morphology of the wings and attachment apparatus in the evolution of the family Hippoboscidae (Diptera)

Using a complex analysis of the molecular genetics, morphological, and ecological characteristics of Hippoboscidae flies, the phylogenetic structure and trends in the evolution of morphological characters that contribute to the ectoparasitic lifestyle of hippoboscid flies of the north of Eurasia were studied for the first time. The research was carried out on 26 Palearctic species from 10 genera. The analysis of molecular phylogeny revealed the levels of clustering of the family with the species predominantly parasitizing mammals or birds, the time of cluster formation, and the divergence of species in the Palearctic conditions. An independent adaptation to birds occurred in the genera Icosta, Pseudolynchia, Ornithoica, and others. Bird parasites are characterized by bifid tarsal claws, long hooks on pulvilli, and long empodium setae (except genus Ornithoica). Mammalian parasites are characterized by simple tarsal claws, short lobes of hooks on pulvilli, and zones on empodium with short setae. Specialization in empodium and pulvillus morphotypes and wing reduction are higher diverged in mammalian parasites than in bird parasites. The decrease of flight ability and wing reduction independently arose in different subfamilies of Hippoboscidae flies. Our results assume that the tribe Ornithomyini is a paraphyletic group, since, according to the complex of morphological features, the genus Ornithoica can be considered a separate lineage of evolution. Hippoboscidae flies have a worldwide distribution and are vectors of many dangerous diseases both in mammals and birds. They have unique morphological adaptations, closely related to their ectoparasitic lifestyles, such as wing characteristics and elements of the tarsi‐attachment apparatus. These features are of great importance in the evolution of this family.