Caffeic Acid Phenethyl Ester (CAPE), a natural polyphenol to increase the therapeutic window for lung adenocarcinomas
•CAPE sensitizes only adenocarcinoma NSCLC to irradiation.•Mechanistically CAPE affects cell cycle distribution, metabolism and NF-κB activity.•CAPE decreases radiation-induced pro-inflammatory markers in ex vivo lung tissue. Lung cancers are highly resistant to radiotherapy, necessitating the use o...
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Veröffentlicht in: | Radiotherapy and oncology 2024-01, Vol.190, p.110021, Article 110021 |
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Sprache: | eng |
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Zusammenfassung: | •CAPE sensitizes only adenocarcinoma NSCLC to irradiation.•Mechanistically CAPE affects cell cycle distribution, metabolism and NF-κB activity.•CAPE decreases radiation-induced pro-inflammatory markers in ex vivo lung tissue.
Lung cancers are highly resistant to radiotherapy, necessitating the use of high doses, which leads to radiation toxicities such as radiation pneumonitis and fibrosis. Caffeic Acid Phenethyl Ester (CAPE) has been suggested to have anti-proliferative and pro-apoptotic effects in tumour cells, while radioprotective anti-inflammatory and anti-oxidant effects in the normal tissue. We investigated the radiosensitizing and radioprotective effects of CAPE in lung cancer cell lines and normal tissue in vitro and ex vivo, respectively.
The cytotoxic and radiosensitizing effects of CAPE in lung cancer were investigated using viability and clonogenic survival assays. The radioprotective effects of CAPE were assessed in vitro and ex vivo using precision cut lung slices (PCLS). Potential underlying molecular mechanisms of CAPE focusing on cell cycle, cell metabolism, mitochondrial function and pro-inflammatory markers were investigated.
Treatment with CAPE decreased cell viability in a dose-dependent manner (IC50 57.6 ± 16.6 μM). Clonogenic survival assays showed significant radiosensitization by CAPE in lung adenocarcinoma lines (p |
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ISSN: | 0167-8140 1879-0887 1879-0887 |
DOI: | 10.1016/j.radonc.2023.110021 |