Fluorescent actinometers for fast and simple quantitative measurement of light intensity

Fluorescent actinometers for fast and simple quantitative measurement of light intensity

Fluorescent actinometers enable the measurement of light intensity even in the depths of samples and over wide ranges of wavelengths and intensities. We introduce two protocols to quantitatively characterize the spatial distribution of light of various fluorescence imaging systems and to calibrate t...

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Veröffentlicht in:Nature methods 2023-12, Vol.20 (12), p.1881-1882
Format: Artikel
Sprache:eng
Schlagworte:
631/1647/245/2225
631/57
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
End users
Fluorescence
Life Sciences
Light
Light intensity
Light sources
Luminous intensity
Microscopy
Photochemistry
Proteomics
Reproducibility
Research Briefing
Spatial distribution
Wavelengths
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Beschreibung
Zusammenfassung:Fluorescent actinometers enable the measurement of light intensity even in the depths of samples and over wide ranges of wavelengths and intensities. We introduce two protocols to quantitatively characterize the spatial distribution of light of various fluorescence imaging systems and to calibrate the illumination of commercially available instruments and light sources.
ISSN:1548-7091
1548-7105
DOI:10.1038/s41592-023-02062-z