Quality of the sample-based RNA determines the real-time RT-PCR results in the laboratory diagnosis of COVID-19
Effect of the quality of sample-based RNA on COVID-19 real-time RT-PCR results was investigated. The purity of the extracts was dependent on the extraction method (P
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2024-01, Vol.108 (1), p.116076-116076, Article 116076 |
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| container_end_page | 116076 |
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| container_title | Diagnostic microbiology and infectious disease |
| container_volume | 108 |
| creator | Shihab, Sibra RM Iqbal, Bushran N. Arunasalam, Shiyamalee Noordeen, Faseeha |
| description | Effect of the quality of sample-based RNA on COVID-19 real-time RT-PCR results was investigated. The purity of the extracts was dependent on the extraction method (P |
| doi_str_mv | 10.1016/j.diagmicrobio.2023.116076 |
| format | Article |
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The purity of the extracts was dependent on the extraction method (P<0.0001) and affected the test interpretations (P = 0.002). Gross RNA concentration negatively correlated with Ct values (P < 0.0001). The presence of impurities contributed to inconclusive test results.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2023.116076</identifier><identifier>PMID: 37980858</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Clinical Laboratory Techniques - methods ; COVID-19 ; COVID-19 - diagnosis ; COVID-19 Testing ; Ct value ; Humans ; Inconclusive results ; Reverse Transcriptase Polymerase Chain Reaction ; RNA concentration ; RNA, Viral - analysis ; RNA, Viral - genetics ; SARS-CoV-2 ; SARS-CoV-2 - genetics ; Sensitivity and Specificity</subject><ispartof>Diagnostic microbiology and infectious disease, 2024-01, Vol.108 (1), p.116076-116076, Article 116076</ispartof><rights>2023 Elsevier Inc.</rights><rights>Copyright © 2023 Elsevier Inc. 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| identifier | ISSN: 0732-8893 |
| ispartof | Diagnostic microbiology and infectious disease, 2024-01, Vol.108 (1), p.116076-116076, Article 116076 |
| issn | 0732-8893 1879-0070 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Clinical Laboratory Techniques - methods COVID-19 COVID-19 - diagnosis COVID-19 Testing Ct value Humans Inconclusive results Reverse Transcriptase Polymerase Chain Reaction RNA concentration RNA, Viral - analysis RNA, Viral - genetics SARS-CoV-2 SARS-CoV-2 - genetics Sensitivity and Specificity |
| title | Quality of the sample-based RNA determines the real-time RT-PCR results in the laboratory diagnosis of COVID-19 |
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