Quality of the sample-based RNA determines the real-time RT-PCR results in the laboratory diagnosis of COVID-19

Effect of the quality of sample-based RNA on COVID-19 real-time RT-PCR results was investigated. The purity of the extracts was dependent on the extraction method (P

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Bibliographische Detailangaben
Veröffentlicht in:	Diagnostic microbiology and infectious disease 2024-01, Vol.108 (1), p.116076-116076, Article 116076
Hauptverfasser:	Shihab, Sibra RM, Iqbal, Bushran N., Arunasalam, Shiyamalee, Noordeen, Faseeha
Format:	Artikel
Sprache:	eng
Schlagworte:	Clinical Laboratory Techniques - methods COVID-19 COVID-19 - diagnosis COVID-19 Testing Ct value Humans Inconclusive results Reverse Transcriptase Polymerase Chain Reaction RNA concentration RNA, Viral - analysis RNA, Viral - genetics SARS-CoV-2 SARS-CoV-2 - genetics Sensitivity and Specificity
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Zusammenfassung:	Effect of the quality of sample-based RNA on COVID-19 real-time RT-PCR results was investigated. The purity of the extracts was dependent on the extraction method (P
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2023.116076