A micro-nano interface integrated SERS-based microfluidic sensor for miRNA detection using DNAzyme walker amplification

Precise and specific miRNA detection plays a vital role in exploring development mechanisms of cancer disease, thereby it can significantly improve relevant prevention and treatment strategies. In this work, a surface-enhanced Raman spectroscopy (SERS)-based microfluidic chip has been devised with a...

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Veröffentlicht in:Analytica chimica acta 2023-12, Vol.1283, p.341957-341957, Article 341957
Hauptverfasser: Lu, Yang, Yu, Yiyue, Wang, Yeru, Zhou, Wenbo, Cheng, Ziyi, Yu, Liandong, Zheng, Shaojiang, Gao, Rongke
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Sprache:eng
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Zusammenfassung:Precise and specific miRNA detection plays a vital role in exploring development mechanisms of cancer disease, thereby it can significantly improve relevant prevention and treatment strategies. In this work, a surface-enhanced Raman spectroscopy (SERS)-based microfluidic chip has been devised with a microcone array SERS substrate (MCASS) for the miR-141 detection. This substrate excels in unique SERS activity and large surface area for DNA oligonucleotide modification. As the presence of miR-141, the DNAzyme walker induced cleavage reaction took place on the finely designed and prepared dual DNA conjugated SERS nanoprobes. The SERS nanoprobes can anchor on MCASS by the DNA hybridization that achieved an impressive detection limit in the femtomolar level. With this integrated SERS-based microfluidic chip, we provided a miRNA detection strategy using DNAzyme walker amplification technology. It is believed that this strategy could be a powerful tool for miRNA detection and related cancer screening test. [Display omitted] •DNAzyme walker induced SERS nanoprobes in microfluidic channel was firstly demonstrated for miRNA detection.•The newly fabricated MCASS featured as SERS substrate and its large surface area that can improve DNA modifications.•The Raman signals of the target were enhanced by MCASS substrates and SERS nanoprobes together.•The miR-141 was successfully detected with high sensitivity and selectivity.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2023.341957