2D and 3D Micropatterning of Mussel‐Inspired Functional Materials by Direct Laser Writing

This work addresses the critical need for multifunctional materials and substrate‐independent high‐precision surface modification techniques that are essential for advancing microdevices and sensing elements. To overcome existing limitations, the versatility of mussel‐inspired materials (MIMs) is combined with state‐of‐the‐art multiphoton direct laser writing (DLW) microfabrication. In this way, 2D and 3D MIM microstructures of complex designs are demonstrated with sub‐micron to micron resolution and extensive post‐functionalization capabilities. This study includes polydopamine (PDA), mussel‐inspired linear, and dendritic polyglycerols (MI‐lPG and MI‐dPG), allowing their direct microstructure on the substrate of choice with the option to tailor the patterned topography and morphology in a controllable manner. The functionality potential of MIMs is demonstrated by successfully immobilizing and detecting single‐stranded DNA on MIM micropattern and nanoarray surfaces. In addition, easy modification of MIM microstructure with silver nanoparticles without the need of any reducing agent is shown. The methodology developed here enables the integration of MIMs in advanced applications where precise surface functionalization is essential. Highly customizable 2D and 3D microstructures with sub‐micron to micron resolution are achieved by combining mussel‐inspired materials (MIMs) with multiphoton lithography microfabrication technique. The presented MIM nano‐ and microstructures can be easily post‐functionalized and are used for DNA detection and electroless metallization with silver nanoparticles. The proposed approach enables precise surface functionalization of choice in various miniaturized systems.