Selecting recombinants to stack high protein with high oleic acid and low linoleic acid in soybean (Glycine max)

Soybean accounts for over a quarter of the world's oilseed consumption and over 70% of the world's protein meal consumption. The separate development of high oleic, low linolenic acid (HOLL) soybean and high‐protein (HP) soybean means that no soybean cultivar on the market has an optimal fatty acid profile and increased protein. The objective of this study was to develop and evaluate high protein, high oleic acid, and low linolenic acid (HP‐HOLL) soybean. A five‐gene stack was created using a two‐phase forward breeding scheme and marker‐assisted selection method. Forty‐six HP‐HOLL lines from three genetic backgrounds were grown in six environments in the Southeast United States. Although genotype‐by‐environment interaction was significant for seed composition traits, lines met the >75% and