Jiella pelagia sp. nov., isolated from the phosphonate-amended seawater of the northwestern Pacific Ocean

A novel Gram-stain-negative, aerobic, rod-shaped bacterium, designated as HL-NP1 T , was isolated from the surface water of the northwestern Pacific Ocean after enrichment cultivation using the organic phosphorous compound of 2-aminoethylphosphonate. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belonged to the genus Jiella , with the highest similarity to Jiella pacifica 40Bstr34 T (98.7 %). The complete genome sequence of strain HL-NP1 T comprised a circular chromosome of 5.58 Mbp and two circular plasmids of 0.15 and 0.22 Mbp. Comparison of the genome sequences between strains HL-NP1 T and J. pacifica 40Bstr34 T revealed that average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values (88.0, 86.4 and 33.9 %, respectively) were below the recommended cut-off levels for delineating bacterial species. Strain HL-NP1 T showed optimal growth at 30 °C, pH 6.5–7.0, with 2.0–2.5 % (w/v) NaCl. The sole respiratory quinone was ubiquinone-10. The predominant fatty acid was summed feature 8 (C 18 : 1 ω 6 c and/or C 18 : 1 ω 7 c ). The polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid and four unidentified lipids. The G+C content of the genomic DNA was 65.1 %. Based on phylogenetic, genotypic, phenotypic and chemotaxonomic data, strain HL-NP1 T is proposed to represent a novel species of the genus Jiella , for which the name Jiella pelagia sp. nov. is proposed. The type strain is HL-NP1 T (= KCCM 90499 T = JCM 35838 T ).