N-glycosylation in Archaea: Unusual sugars and unique modifications

Archaea are microorganisms that comprise a distinct branch of the universal tree of life and which are best known as extremophiles, residing in a variety of environments characterized by harsh physical conditions. One seemingly universal trait of Archaea is the ability to perform N-glycosylation. At the same time, archaeal N-linked glycans present variety in terms of both composition and architecture not seen in the parallel eukaryal or bacterial processes. In this mini-review, many of the unique and unusual sugars found in archaeal N-linked glycans as identified by nuclear magnetic resonance spectroscopy are described. [Display omitted] •N-glycosylation is an almost universal event in Archaea.•Archaeal N-glycosylation presents diversity not seen elsewhere.•Archaeal N-linked glycans incorporate unique and unusual sugars.