Predation-resistant Pseudomonas bacteria engage in symbiont-like behavior with the social amoeba Dictyostelium discoideum
The soil amoeba Dictyostelium discoideum acts as both a predator and potential host for diverse bacteria. We tested fifteen Pseudomonas strains that were isolated from transiently infected wild D. discoideum for ability to escape predation and infect D. discoideum fruiting bodies. Three predation-re...
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Veröffentlicht in: | The ISME Journal 2023-12, Vol.17 (12), p.2352-2361 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The soil amoeba
Dictyostelium discoideum
acts as both a predator and potential host for diverse bacteria. We tested fifteen
Pseudomonas
strains that were isolated from transiently infected wild
D. discoideum
for ability to escape predation and infect
D. discoideum
fruiting bodies. Three predation-resistant strains frequently caused extracellular infections of fruiting bodies but were not found within spores. Furthermore, infection by one of these species induces secondary infections and suppresses predation of otherwise edible bacteria. Another strain can persist inside of amoebae after being phagocytosed but is rarely taken up. We sequenced isolate genomes and discovered that predation-resistant isolates are not monophyletic. Many
Pseudomonas
isolates encode secretion systems and toxins known to improve resistance to phagocytosis in other species, as well as diverse secondary metabolite biosynthetic gene clusters that may contribute to predation resistance. However, the distribution of these genes alone cannot explain why some strains are edible and others are not. Each lineage may employ a unique mechanism for resistance. |
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ISSN: | 1751-7362 1751-7370 |
DOI: | 10.1038/s41396-023-01535-5 |