A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

As one of the important organelles in cells, lipid droplets (LDs) are involved in various physiological processes, especially affecting the occurrence and progression of non-alcoholic fatty liver (NAFL). Therefore, it is of great significance to develop LD-specific probes with excellent biocompatibility, deep penetration and bright fluorescence. Herein, a fluorescent probe LD-HWZ was designed and synthesized based on triphenylamine and the dicyanoisophorone group. It is found that probe LD-HWZ has a large Stokes shift (Δ λ = 160 nm in DMSO) and exhibits bright fluorescence in a lipid environment. In addition, biological experiments showed that LD-HWZ can localize in lipid droplets, which can be used to detect the dynamic changes of LDs. Importantly, LD-HWZ has been successfully used to discriminate NAFL tissues from normal livers. The excellent properties of probe LD-HWZ in this work are expected to shed new light on the design of lipid droplet probes for the study of fatty liver diagnosis. Probe LD-HWZ with large Stokes shifts enables dynamic monitoring LDs via wash-free procedure, effectively distinguishing between fatty liver and normal liver. This finding holds great promise for developing novel materials for NAFL diagnosis.