Exploring the Role of Metal in the Biointeraction of Metallacarboranes with C. elegans Embryos

Cobaltabis(dicarbollides), ferrabis(dicarbollide), and their halogenated derivatives are the most studied metallacarboranes with great medical potential. These versatile compounds and their iodinated derivatives can be used in chemotherapy, radiotherapy, particle therapy, and bioimaging when isotopes are used. These metallacarboranes have been evaluated in vitro and recently in vivo with complex animal models. Lately, these studies have been complemented using the invertebrate Caenorhabditis elegans (C. elegans), a nematode largely used in toxicology. When evaluated at the L4 stage, cobaltabis(dicarbollides), ([o‐COSAN]− and [8,8’‐I2‐o‐COSAN]−), exhibited a higher mean lethal dose (LD50) than ferrabis(dicarbollides) ([o‐FESAN]− and [8,8’‐I2‐o‐FESAN]−). In this work, we used the C. elegans embryos since they are a complex biological barrier with concentric layers of polysaccharides and proteins that protect them from the environment. We assessed if the metal atom changes their biointeraction with the C. elegans embryos. First, we assessed the effects on embryo development for metallacarboranes and their di‐iodinated derivatives. We observed changes in color and in their surface structure. An exhaustive physicochemical characterization was performed to understand better this interaction, revealing a stronger interaction of ferrabis(dicarbollide) compounds with C. elegans embryos than the cobaltabis(dicarbollide) molecules. Unveiling the biological interaction of these compounds is of great interest for their future biomedical applications. We have transitioned from performing chemical scale studies involving the interaction of metallabis(dicarbollides) with biomolecules in a round‐bottom flask, to now conducting in vivo experiments using C. elegans. Results suggest metal (Fe or Co) plays a crucial role in C. elegans at the L4 stage and in embryos, and shows a stronger interaction with ferra compared to cobaltabis(dicarbollides).