Plasmonic inhibition of bacterial adhesion on gold-decorated mesoporous zirconium oxide thin films

Preventing bacterial development on surfaces is essential to avoid problems caused by biofouling. Surfaces decorated with gold nanoparticles have been shown to thermally kill bacteria under high-intensity NIR illumination. In this study, we evaluated the colonization by E. coli of nanostructured sur...

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Veröffentlicht in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2023-12, Vol.232, p.113576-113576, Article 113576
Hauptverfasser: Florencia Tebele, M., Paris, Gastón, Zelcer, Andrés
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Sprache:eng
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Zusammenfassung:Preventing bacterial development on surfaces is essential to avoid problems caused by biofouling. Surfaces decorated with gold nanoparticles have been shown to thermally kill bacteria under high-intensity NIR illumination. In this study, we evaluated the colonization by E. coli of nanostructured surfaces composed of mesoporous zirconia thin films, both with and without gold nanoparticles embedded into the pores. We studied the effect of the nanostructure and of low intensity visible light excitation of the gold nanoparticles on the colonization process. We found that neither the zirconia, nor the presence of pores, or even gold nanoparticles affect bacterial adhesion compared to the bare glass substrate. Therefore, mesoporous zirconia thin films are biologically inert scaffolds that enable the construction of robust surfaces containing functional nanoparticles that can affect bacterial growth. When the gold containing surfaces are irradiated with light, bacterial adhesion shows a remarkable 96±4% reduction. Our studies revealed that these surfaces affect early colonization steps, prior to biofilm formation, preventing bacterial adhesion without affecting its viability. In contrast to related systems where plasmonic excitation induces membrane damage due to strong local heating, the membrane integrity is preserved, showing that these surfaces have a different working principle. [Display omitted] •Neither dense, mesoporous, and gold-decorated mesoporous ZrO2 affect E. coli colonization.•Excitation of gold nanoparticle’s plasmon using low-intensity light prevents E. coli adhesion.•Plasmonic excitation with low intensity light does not detach or damage bacteria already adhered to the surface.•The antiadhesion mechanism does not involve cell membrane rupture by photothermal effect.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2023.113576