Monolithically integrated semiconductor fluorescence sensor for microfluidic applications
This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773 nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These opto...
Gespeichert in:
Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2005-03, Vol.105 (2), p.393-399 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 399 |
---|---|
container_issue | 2 |
container_start_page | 393 |
container_title | Sensors and actuators. B, Chemical |
container_volume | 105 |
creator | Thrush, Evan Levi, Ofer Cook, Laura J. Deich, Jason Kurtz, Andrea Smith, Stephen J. Moerner, W.E. Harris, James S. |
description | This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773
nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These optoelectronic components are optically coupled to a glass microfluidic channel (100
μm width and 45
μm depth) through the use of a discrete micro-lens to form a complete sensor. The experimental limit of detection was 250
nM of IRDye 800 Phosphoramidite. Based on an S/N = 3, the theoretical limit of detection was determined to be 40
nM. Laser background levels currently limit the sensor sensitivity. Large gains in sensitivity are possible through the systematic reduction of laser background by increasing spectral and spatial filtration. The low-cost, compact and parallel architecture makes this sensor a candidate for fluorescence-based sensing applications. |
doi_str_mv | 10.1016/j.snb.2004.06.028 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_28795614</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400504004666</els_id><sourcerecordid>28795614</sourcerecordid><originalsourceid>FETCH-LOGICAL-c425t-713fab059e3c85b43da78bf639210d4f8c07250419f6a343d234440a1a6f73f63</originalsourceid><addsrcrecordid>eNqNkM1OxCAURonRxHH0Adx15a71Uii0cWUm_iVj3OjCFaGUKhMGKjAm8_bSjGvjgpDwne-GexC6xFBhwOx6U0XXVzUArYBVULdHaIFbTkoCnB-jBXR1U1KA5hSdxbiBDBIGC_T-7J23Jn0aJa3dF8Yl_RFk0kMR9dYo74adSj4Uo935oKPSTukcuTi_5ZOZ4HNoBqMKOU02D0rGu3iOTkZpo774vZfo7f7udfVYrl8enla361LRukklx2SUPTSdJqptekoGydt-ZKSrMQx0bBXwugGKu5FJkuOaUEpBYslGTjK3RFeHuVPwXzsdk9ia_E1rpdN-F0Xd8q5hmP4DzBinkEF8APNmMQY9iimYrQx7gUHMtsVGZNtiti2AiWw7d24OHZ1X_TY6iKjM7GowQaskBm_-aP8AsWaJUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>28561740</pqid></control><display><type>article</type><title>Monolithically integrated semiconductor fluorescence sensor for microfluidic applications</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Thrush, Evan ; Levi, Ofer ; Cook, Laura J. ; Deich, Jason ; Kurtz, Andrea ; Smith, Stephen J. ; Moerner, W.E. ; Harris, James S.</creator><creatorcontrib>Thrush, Evan ; Levi, Ofer ; Cook, Laura J. ; Deich, Jason ; Kurtz, Andrea ; Smith, Stephen J. ; Moerner, W.E. ; Harris, James S.</creatorcontrib><description>This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773
nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These optoelectronic components are optically coupled to a glass microfluidic channel (100
μm width and 45
μm depth) through the use of a discrete micro-lens to form a complete sensor. The experimental limit of detection was 250
nM of IRDye 800 Phosphoramidite. Based on an S/N = 3, the theoretical limit of detection was determined to be 40
nM. Laser background levels currently limit the sensor sensitivity. Large gains in sensitivity are possible through the systematic reduction of laser background by increasing spectral and spatial filtration. The low-cost, compact and parallel architecture makes this sensor a candidate for fluorescence-based sensing applications.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2004.06.028</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Fluorescence ; Infrared dye ; Microfluidics ; Near-infrared ; Optical sensor ; Photodetectors ; Vertical-cavity surface-emitting laser (VCSEL)</subject><ispartof>Sensors and actuators. B, Chemical, 2005-03, Vol.105 (2), p.393-399</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-713fab059e3c85b43da78bf639210d4f8c07250419f6a343d234440a1a6f73f63</citedby><cites>FETCH-LOGICAL-c425t-713fab059e3c85b43da78bf639210d4f8c07250419f6a343d234440a1a6f73f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925400504004666$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids></links><search><creatorcontrib>Thrush, Evan</creatorcontrib><creatorcontrib>Levi, Ofer</creatorcontrib><creatorcontrib>Cook, Laura J.</creatorcontrib><creatorcontrib>Deich, Jason</creatorcontrib><creatorcontrib>Kurtz, Andrea</creatorcontrib><creatorcontrib>Smith, Stephen J.</creatorcontrib><creatorcontrib>Moerner, W.E.</creatorcontrib><creatorcontrib>Harris, James S.</creatorcontrib><title>Monolithically integrated semiconductor fluorescence sensor for microfluidic applications</title><title>Sensors and actuators. B, Chemical</title><description>This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773
nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These optoelectronic components are optically coupled to a glass microfluidic channel (100
μm width and 45
μm depth) through the use of a discrete micro-lens to form a complete sensor. The experimental limit of detection was 250
nM of IRDye 800 Phosphoramidite. Based on an S/N = 3, the theoretical limit of detection was determined to be 40
nM. Laser background levels currently limit the sensor sensitivity. Large gains in sensitivity are possible through the systematic reduction of laser background by increasing spectral and spatial filtration. The low-cost, compact and parallel architecture makes this sensor a candidate for fluorescence-based sensing applications.</description><subject>Fluorescence</subject><subject>Infrared dye</subject><subject>Microfluidics</subject><subject>Near-infrared</subject><subject>Optical sensor</subject><subject>Photodetectors</subject><subject>Vertical-cavity surface-emitting laser (VCSEL)</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqNkM1OxCAURonRxHH0Adx15a71Uii0cWUm_iVj3OjCFaGUKhMGKjAm8_bSjGvjgpDwne-GexC6xFBhwOx6U0XXVzUArYBVULdHaIFbTkoCnB-jBXR1U1KA5hSdxbiBDBIGC_T-7J23Jn0aJa3dF8Yl_RFk0kMR9dYo74adSj4Uo935oKPSTukcuTi_5ZOZ4HNoBqMKOU02D0rGu3iOTkZpo774vZfo7f7udfVYrl8enla361LRukklx2SUPTSdJqptekoGydt-ZKSrMQx0bBXwugGKu5FJkuOaUEpBYslGTjK3RFeHuVPwXzsdk9ia_E1rpdN-F0Xd8q5hmP4DzBinkEF8APNmMQY9iimYrQx7gUHMtsVGZNtiti2AiWw7d24OHZ1X_TY6iKjM7GowQaskBm_-aP8AsWaJUA</recordid><startdate>20050328</startdate><enddate>20050328</enddate><creator>Thrush, Evan</creator><creator>Levi, Ofer</creator><creator>Cook, Laura J.</creator><creator>Deich, Jason</creator><creator>Kurtz, Andrea</creator><creator>Smith, Stephen J.</creator><creator>Moerner, W.E.</creator><creator>Harris, James S.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>H8D</scope></search><sort><creationdate>20050328</creationdate><title>Monolithically integrated semiconductor fluorescence sensor for microfluidic applications</title><author>Thrush, Evan ; Levi, Ofer ; Cook, Laura J. ; Deich, Jason ; Kurtz, Andrea ; Smith, Stephen J. ; Moerner, W.E. ; Harris, James S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-713fab059e3c85b43da78bf639210d4f8c07250419f6a343d234440a1a6f73f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Fluorescence</topic><topic>Infrared dye</topic><topic>Microfluidics</topic><topic>Near-infrared</topic><topic>Optical sensor</topic><topic>Photodetectors</topic><topic>Vertical-cavity surface-emitting laser (VCSEL)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thrush, Evan</creatorcontrib><creatorcontrib>Levi, Ofer</creatorcontrib><creatorcontrib>Cook, Laura J.</creatorcontrib><creatorcontrib>Deich, Jason</creatorcontrib><creatorcontrib>Kurtz, Andrea</creatorcontrib><creatorcontrib>Smith, Stephen J.</creatorcontrib><creatorcontrib>Moerner, W.E.</creatorcontrib><creatorcontrib>Harris, James S.</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Aerospace Database</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thrush, Evan</au><au>Levi, Ofer</au><au>Cook, Laura J.</au><au>Deich, Jason</au><au>Kurtz, Andrea</au><au>Smith, Stephen J.</au><au>Moerner, W.E.</au><au>Harris, James S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monolithically integrated semiconductor fluorescence sensor for microfluidic applications</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2005-03-28</date><risdate>2005</risdate><volume>105</volume><issue>2</issue><spage>393</spage><epage>399</epage><pages>393-399</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773
nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These optoelectronic components are optically coupled to a glass microfluidic channel (100
μm width and 45
μm depth) through the use of a discrete micro-lens to form a complete sensor. The experimental limit of detection was 250
nM of IRDye 800 Phosphoramidite. Based on an S/N = 3, the theoretical limit of detection was determined to be 40
nM. Laser background levels currently limit the sensor sensitivity. Large gains in sensitivity are possible through the systematic reduction of laser background by increasing spectral and spatial filtration. The low-cost, compact and parallel architecture makes this sensor a candidate for fluorescence-based sensing applications.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2004.06.028</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0925-4005 |
ispartof | Sensors and actuators. B, Chemical, 2005-03, Vol.105 (2), p.393-399 |
issn | 0925-4005 1873-3077 |
language | eng |
recordid | cdi_proquest_miscellaneous_28795614 |
source | Elsevier ScienceDirect Journals Complete |
subjects | Fluorescence Infrared dye Microfluidics Near-infrared Optical sensor Photodetectors Vertical-cavity surface-emitting laser (VCSEL) |
title | Monolithically integrated semiconductor fluorescence sensor for microfluidic applications |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T03%3A21%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Monolithically%20integrated%20semiconductor%20fluorescence%20sensor%20for%20microfluidic%20applications&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Thrush,%20Evan&rft.date=2005-03-28&rft.volume=105&rft.issue=2&rft.spage=393&rft.epage=399&rft.pages=393-399&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2004.06.028&rft_dat=%3Cproquest_cross%3E28795614%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=28561740&rft_id=info:pmid/&rft_els_id=S0925400504004666&rfr_iscdi=true |