NOTCH1 and PIK3CA mutation are related to HPV-associated vulvar squamous cell carcinoma
NOTCH1 and PIK3CA are members of important cell signalling pathways that are deregulated in squamous cell carcinomas of various organs. Vulvar squamous cell carcinomas (vulvSCC) are classically divided into two pathways, HPV-associated or HPV-independent, but the effect of NOTCH1 and PIK3CA mutation...
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| Veröffentlicht in: | Pathology, research and practice research and practice, 2023-11, Vol.251, p.154877-154877, Article 154877 |
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| container_title | Pathology, research and practice |
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| creator | Choschzick, M. Stergiou, C. Gut, A. Zoche, M. Ross, JS Moch, H. |
| description | NOTCH1 and PIK3CA are members of important cell signalling pathways that are deregulated in squamous cell carcinomas of various organs. Vulvar squamous cell carcinomas (vulvSCC) are classically divided into two pathways, HPV-associated or HPV-independent, but the effect of NOTCH1 and PIK3CA mutations in both groups is unclear. We analysed two different cohorts of vulvSCC using Hybrid Capture-based Comprehensive Genomic Profiling and identified NOTCH1 and PIK3CA mutations in 35% and 31% of 48 primary vulvSCC. In this first cohort, PIK3CA and NOTCH1 mutations were significantly correlated with HPV infection (p |
| doi_str_mv | 10.1016/j.prp.2023.154877 |
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Vulvar squamous cell carcinomas (vulvSCC) are classically divided into two pathways, HPV-associated or HPV-independent, but the effect of NOTCH1 and PIK3CA mutations in both groups is unclear. We analysed two different cohorts of vulvSCC using Hybrid Capture-based Comprehensive Genomic Profiling and identified NOTCH1 and PIK3CA mutations in 35% and 31% of 48 primary vulvSCC. In this first cohort, PIK3CA and NOTCH1 mutations were significantly correlated with HPV infection (p < 0.01). Furthermore, mutations in both genes were associated with an advanced tumor stage and poorly differentiated status (p < 0.05). PIK3CA and NOTCH1 mutations were also associated with shorter patient survival which did not reach significance. In the second cohort of 735 advanced vulvSCC from metastatic site biopsies or from sites of unresectable loco-regional disease, NOTCH1 and PIK3CA mutations were reported in 14% and 20.3%, respectively. 4 of 48 (8%) and 22 of 735 vulvSCC (3.0%) featured genomic alterations (short variants and/or copy number changes and/or rearrangements) in both NOTCH1 and PIK3CA. NOTCH1 mutations were mostly located in the extracellular EGF-like domains, were inactivating and indicated that NOTCH1 functions predominantly as a tumor suppressor gene in vulvSCC. In contrast, PIK3CA mutations favored hotspot codons 1624 and 1633 of the gene, indicating that PIK3CA acts as an oncogene in vulvar carcinogenesis. In conclusion, NOTCH1 and PIK3CA mutations are detectable in a substantial proportion of vulvSCC and are related to HPV infection and more aggressive tumor behaviour.</description><identifier>ISSN: 0344-0338</identifier><identifier>EISSN: 1618-0631</identifier><identifier>DOI: 10.1016/j.prp.2023.154877</identifier><language>eng</language><publisher>Elsevier GmbH</publisher><subject>Mutation ; NOTCH1 ; PIK3CA ; Vulvar cancer</subject><ispartof>Pathology, research and practice, 2023-11, Vol.251, p.154877-154877, Article 154877</ispartof><rights>2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-997dd78e146081647e9d04dd6b7259b415cdfb97ea376a5ef46659fabc351d7e3</citedby><cites>FETCH-LOGICAL-c330t-997dd78e146081647e9d04dd6b7259b415cdfb97ea376a5ef46659fabc351d7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.prp.2023.154877$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids></links><search><creatorcontrib>Choschzick, M.</creatorcontrib><creatorcontrib>Stergiou, C.</creatorcontrib><creatorcontrib>Gut, A.</creatorcontrib><creatorcontrib>Zoche, M.</creatorcontrib><creatorcontrib>Ross, JS</creatorcontrib><creatorcontrib>Moch, H.</creatorcontrib><title>NOTCH1 and PIK3CA mutation are related to HPV-associated vulvar squamous cell carcinoma</title><title>Pathology, research and practice</title><description>NOTCH1 and PIK3CA are members of important cell signalling pathways that are deregulated in squamous cell carcinomas of various organs. Vulvar squamous cell carcinomas (vulvSCC) are classically divided into two pathways, HPV-associated or HPV-independent, but the effect of NOTCH1 and PIK3CA mutations in both groups is unclear. We analysed two different cohorts of vulvSCC using Hybrid Capture-based Comprehensive Genomic Profiling and identified NOTCH1 and PIK3CA mutations in 35% and 31% of 48 primary vulvSCC. In this first cohort, PIK3CA and NOTCH1 mutations were significantly correlated with HPV infection (p < 0.01). Furthermore, mutations in both genes were associated with an advanced tumor stage and poorly differentiated status (p < 0.05). PIK3CA and NOTCH1 mutations were also associated with shorter patient survival which did not reach significance. In the second cohort of 735 advanced vulvSCC from metastatic site biopsies or from sites of unresectable loco-regional disease, NOTCH1 and PIK3CA mutations were reported in 14% and 20.3%, respectively. 4 of 48 (8%) and 22 of 735 vulvSCC (3.0%) featured genomic alterations (short variants and/or copy number changes and/or rearrangements) in both NOTCH1 and PIK3CA. NOTCH1 mutations were mostly located in the extracellular EGF-like domains, were inactivating and indicated that NOTCH1 functions predominantly as a tumor suppressor gene in vulvSCC. In contrast, PIK3CA mutations favored hotspot codons 1624 and 1633 of the gene, indicating that PIK3CA acts as an oncogene in vulvar carcinogenesis. In conclusion, NOTCH1 and PIK3CA mutations are detectable in a substantial proportion of vulvSCC and are related to HPV infection and more aggressive tumor behaviour.</description><subject>Mutation</subject><subject>NOTCH1</subject><subject>PIK3CA</subject><subject>Vulvar cancer</subject><issn>0344-0338</issn><issn>1618-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kEtPwzAQhC0EEqXwA7j5yCVlHTt2Ik5VBbSioj3wOFquvZFc5YWdVOLfk1LOnFYazbc7O4TcMpgxYPJ-P-tCN0sh5TOWiVypMzJhkuUJSM7OyQS4EAlwnl-Sqxj3AKBAsAn5fN28LZaMmsbR7eqFL-a0HnrT-7ahJiANWJkeHe1butx-JCbG1vpf5TBUBxNo_BpM3Q6RWqwqak2wvmlrc00uSlNFvPmbU_L-9DheStab59Vivk4s59AnRaGcUzkyISFnUigsHAjn5E6lWbETLLOu3BUKDVfSZFgKKbOiNDvLM-YU8im5O-3tQvs1YOx17eMximlwTKXTXOWQQg5itLKT1YY2xoCl7oKvTfjWDPSxRL0flU4fS9SnEkfm4cTg-MPBY9DRemwsOh_Q9tq1_h_6B4s8eQI</recordid><startdate>202311</startdate><enddate>202311</enddate><creator>Choschzick, M.</creator><creator>Stergiou, C.</creator><creator>Gut, A.</creator><creator>Zoche, M.</creator><creator>Ross, JS</creator><creator>Moch, H.</creator><general>Elsevier GmbH</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202311</creationdate><title>NOTCH1 and PIK3CA mutation are related to HPV-associated vulvar squamous cell carcinoma</title><author>Choschzick, M. ; Stergiou, C. ; Gut, A. ; Zoche, M. ; Ross, JS ; Moch, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-997dd78e146081647e9d04dd6b7259b415cdfb97ea376a5ef46659fabc351d7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Mutation</topic><topic>NOTCH1</topic><topic>PIK3CA</topic><topic>Vulvar cancer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choschzick, M.</creatorcontrib><creatorcontrib>Stergiou, C.</creatorcontrib><creatorcontrib>Gut, A.</creatorcontrib><creatorcontrib>Zoche, M.</creatorcontrib><creatorcontrib>Ross, JS</creatorcontrib><creatorcontrib>Moch, H.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology, research and practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choschzick, M.</au><au>Stergiou, C.</au><au>Gut, A.</au><au>Zoche, M.</au><au>Ross, JS</au><au>Moch, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NOTCH1 and PIK3CA mutation are related to HPV-associated vulvar squamous cell carcinoma</atitle><jtitle>Pathology, research and practice</jtitle><date>2023-11</date><risdate>2023</risdate><volume>251</volume><spage>154877</spage><epage>154877</epage><pages>154877-154877</pages><artnum>154877</artnum><issn>0344-0338</issn><eissn>1618-0631</eissn><abstract>NOTCH1 and PIK3CA are members of important cell signalling pathways that are deregulated in squamous cell carcinomas of various organs. Vulvar squamous cell carcinomas (vulvSCC) are classically divided into two pathways, HPV-associated or HPV-independent, but the effect of NOTCH1 and PIK3CA mutations in both groups is unclear. We analysed two different cohorts of vulvSCC using Hybrid Capture-based Comprehensive Genomic Profiling and identified NOTCH1 and PIK3CA mutations in 35% and 31% of 48 primary vulvSCC. In this first cohort, PIK3CA and NOTCH1 mutations were significantly correlated with HPV infection (p < 0.01). Furthermore, mutations in both genes were associated with an advanced tumor stage and poorly differentiated status (p < 0.05). PIK3CA and NOTCH1 mutations were also associated with shorter patient survival which did not reach significance. In the second cohort of 735 advanced vulvSCC from metastatic site biopsies or from sites of unresectable loco-regional disease, NOTCH1 and PIK3CA mutations were reported in 14% and 20.3%, respectively. 4 of 48 (8%) and 22 of 735 vulvSCC (3.0%) featured genomic alterations (short variants and/or copy number changes and/or rearrangements) in both NOTCH1 and PIK3CA. NOTCH1 mutations were mostly located in the extracellular EGF-like domains, were inactivating and indicated that NOTCH1 functions predominantly as a tumor suppressor gene in vulvSCC. In contrast, PIK3CA mutations favored hotspot codons 1624 and 1633 of the gene, indicating that PIK3CA acts as an oncogene in vulvar carcinogenesis. In conclusion, NOTCH1 and PIK3CA mutations are detectable in a substantial proportion of vulvSCC and are related to HPV infection and more aggressive tumor behaviour.</abstract><pub>Elsevier GmbH</pub><doi>10.1016/j.prp.2023.154877</doi><tpages>1</tpages></addata></record> |
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| subjects | Mutation NOTCH1 PIK3CA Vulvar cancer |
| title | NOTCH1 and PIK3CA mutation are related to HPV-associated vulvar squamous cell carcinoma |
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