CRISPR/Cas12a and primer-assisted rolling circle amplification integrated ultra-sensitive dual-signal sensing platform for EGFR 19 detection

Herein, we integrated CRISPR/Cas12a with primer-assisted rolling circle amplification (PARCA) to specifically detect EGFR 19 from the genome. We fused the method into fluorescent and electrochemical detection systems forming a stable and sensitive dual-signal sensing platform. The fluorescent detection system stably detected EGFR 19 in a linear range from 500 fM to 10 nM with an ultra-low background signal. The electrochemical detection system possessed a detection limit as low as 42 aM due to the introduction of nanomaterial UIO-66-NH2. The dual-signal sensing platform showed superior performance in complex serum samples and real cell genomes and provided a flexible and dynamic approach for the ultra-sensitive detection of EGFR 19. [Display omitted] •Double signal enhancement coupling RCA with CRISPR/Cas12a to EGFR 19 detection.•Flexible fluorescent-electrochemical dual-signal sensor platform.•An ultra-low fluorescent background.•UIO-66-NH2 mediated electrochemical detection limit down to 42 aM.•Excellent application in complex serum samples and real cell genomes.