Involvement of cyclic nucleotide-gated channels in soybean cyst nematode chemotaxis and thermotaxis

The soybean cyst nematode (SCN) is one of the most damaging pests affecting soybean production. SCN displays important host recognition behaviors, such as hatching and infection, by recognizing several compounds produced by the host. Therefore, controlling SCN behaviors such as chemotaxis and thermotaxis is an attractive pest control strategy. In this study, we found that cyclic nucleotide-gated channels (CNG channels) regulate SCN chemotaxis and thermotaxis and Hg-tax-2, a gene encoding a CNG channel, is an important regulator of SCN behavior. Gene silencing of Hg-tax-2 and treatment with a CNG channel inhibitor reduced the attraction of second-stage juveniles to nitrate, an attractant with a different recognition mechanism from the host-derived chemoattractant(s), and to host soybean roots, as well as their avoidance behavior toward high temperatures. Co-treatment of ds Hg-tax-2 with the CNG channel inhibitor indicated that Hg-tax-2 is a major regulator of SCN chemotaxis and thermotaxis. These results suggest new avenues for research on control of SCN. [Display omitted] •The gene Hg-tax-2 was identified in soybean cyst nematode (SCN).•Hg-tax-2 encodes cyclic nucleotide-gated channels (CNG channels).•RNAi of Hg-tax-2 disrupted SCN chemotaxis and thermotaxis.•CNG channel inhibitors disrupted SCN chemotaxis and thermotaxis.•Among CNG channels, Hg-tax-2 is a major regulator of SCN behavior.