3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans
Purpose To develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases. Materials and Methods TiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercial...
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| Veröffentlicht in: | Journal of prosthodontics 2024-09, Vol.33 (S1), p.25-34 |
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| container_title | Journal of prosthodontics |
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| creator | Altarazi, Ahmed Jadaan, Layali McBain, Andrew J. Haider, Julfikar Kushnerev, Evgeny Yates, Julian M. Alhotan, Abdulaziz Silikas, Nick Devlin, Hugh |
| description | Purpose
To develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases.
Materials and Methods
TiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercially available 3D‐printed resin material. The resulting nanocomposite material was analyzed using Lactate dehydrogenase (LDH) and AlamarBlue (AB) assays for biocompatibility testing with human gingival fibroblasts (HGF). The composite material was also tested for its antifungal efficacy against Candida albicans. Fourier transform infrared (FTIR) and Energy Dispersive X‐ray Spectroscopy (EDX) mapping were conducted to assess the surface coating and the dispersion of the NPs.
Results
LDH and AB assays confirmed the biocompatibility of the material showing cell proliferation at a rate of nearly 100% at day 10, with a cytotoxicity of less than 13% of the cells at day 10. The concentrations of 0.10, 0.25, and 0.50 wt.% caused a significant reduction (p |
| doi_str_mv | 10.1111/jopr.13784 |
| format | Article |
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To develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases.
Materials and Methods
TiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercially available 3D‐printed resin material. The resulting nanocomposite material was analyzed using Lactate dehydrogenase (LDH) and AlamarBlue (AB) assays for biocompatibility testing with human gingival fibroblasts (HGF). The composite material was also tested for its antifungal efficacy against Candida albicans. Fourier transform infrared (FTIR) and Energy Dispersive X‐ray Spectroscopy (EDX) mapping were conducted to assess the surface coating and the dispersion of the NPs.
Results
LDH and AB assays confirmed the biocompatibility of the material showing cell proliferation at a rate of nearly 100% at day 10, with a cytotoxicity of less than 13% of the cells at day 10. The concentrations of 0.10, 0.25, and 0.50 wt.% caused a significant reduction (p < 0.05) in the number of candida cells attached to the surface of the specimens (p < 0.05), while 0.75 wt.% did not show any significant difference compared to the control (no TiO2 NPs) (p > 0.05). FTIR and EDX analysis confirmed the presence of TiO2 NPs within the nanocomposite material with a homogenous dispersion for 0.10 and 0.25 wt.% groups and an aggregation of the NPs within the material at higher concentrations.
Conclusion
The addition of TiO2 NPs into 3D‐printed denture base resin proved to have an antifungal effect against Candida albicans. The resultant nanocomposite material was a biocompatible material with HGFs and was successfully used for 3D printing.</description><identifier>ISSN: 1059-941X</identifier><identifier>ISSN: 1532-849X</identifier><identifier>EISSN: 1532-849X</identifier><identifier>DOI: 10.1111/jopr.13784</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc</publisher><subject>additive manufacturing ; antifungal ; Antifungal activity ; Biocompatibility ; Candida albicans ; Cell proliferation ; Composite materials ; Cytotoxicity ; denture base resin ; Dentures ; Fourier transforms ; L-Lactate dehydrogenase ; Nanocomposites ; Nanoparticles ; Resins ; Titanium dioxide</subject><ispartof>Journal of prosthodontics, 2024-09, Vol.33 (S1), p.25-34</ispartof><rights>2023 The Authors. published by Wiley Periodicals LLC on behalf of American College of Prosthodontists.</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 The Authors. Journal of Prosthodontics published by Wiley Periodicals LLC on behalf of American College of Prosthodontists.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-0537-8884 ; 0000-0001-7010-8285</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjopr.13784$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjopr.13784$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Altarazi, Ahmed</creatorcontrib><creatorcontrib>Jadaan, Layali</creatorcontrib><creatorcontrib>McBain, Andrew J.</creatorcontrib><creatorcontrib>Haider, Julfikar</creatorcontrib><creatorcontrib>Kushnerev, Evgeny</creatorcontrib><creatorcontrib>Yates, Julian M.</creatorcontrib><creatorcontrib>Alhotan, Abdulaziz</creatorcontrib><creatorcontrib>Silikas, Nick</creatorcontrib><creatorcontrib>Devlin, Hugh</creatorcontrib><title>3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans</title><title>Journal of prosthodontics</title><description>Purpose
To develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases.
Materials and Methods
TiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercially available 3D‐printed resin material. The resulting nanocomposite material was analyzed using Lactate dehydrogenase (LDH) and AlamarBlue (AB) assays for biocompatibility testing with human gingival fibroblasts (HGF). The composite material was also tested for its antifungal efficacy against Candida albicans. Fourier transform infrared (FTIR) and Energy Dispersive X‐ray Spectroscopy (EDX) mapping were conducted to assess the surface coating and the dispersion of the NPs.
Results
LDH and AB assays confirmed the biocompatibility of the material showing cell proliferation at a rate of nearly 100% at day 10, with a cytotoxicity of less than 13% of the cells at day 10. The concentrations of 0.10, 0.25, and 0.50 wt.% caused a significant reduction (p < 0.05) in the number of candida cells attached to the surface of the specimens (p < 0.05), while 0.75 wt.% did not show any significant difference compared to the control (no TiO2 NPs) (p > 0.05). FTIR and EDX analysis confirmed the presence of TiO2 NPs within the nanocomposite material with a homogenous dispersion for 0.10 and 0.25 wt.% groups and an aggregation of the NPs within the material at higher concentrations.
Conclusion
The addition of TiO2 NPs into 3D‐printed denture base resin proved to have an antifungal effect against Candida albicans. The resultant nanocomposite material was a biocompatible material with HGFs and was successfully used for 3D printing.</description><subject>additive manufacturing</subject><subject>antifungal</subject><subject>Antifungal activity</subject><subject>Biocompatibility</subject><subject>Candida albicans</subject><subject>Cell proliferation</subject><subject>Composite materials</subject><subject>Cytotoxicity</subject><subject>denture base resin</subject><subject>Dentures</subject><subject>Fourier transforms</subject><subject>L-Lactate dehydrogenase</subject><subject>Nanocomposites</subject><subject>Nanoparticles</subject><subject>Resins</subject><subject>Titanium dioxide</subject><issn>1059-941X</issn><issn>1532-849X</issn><issn>1532-849X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><recordid>eNpdkU1OwzAQhSMEElDYcAJLbNikxD9JbHao_AupCBWpO8txxuAqtYPtCrGCI3BGTkJaWDGbmcX3nkbvZdkRLsZ4mNOF78MY05qzrWwPl5TknIn59nAXpcgFw_PdbD_GRVFgXHK8l33Qi-_Prz5Yl6BFTjmv_bL30SZALbi0CoAaFQEFiNadodkLIDAGdELeIOu0D70PKln3jGZ2SjYWvQrJ6g4i8g6lQfEc_Ft6WSsmyrW2VUh1jdXKxYNsx6guwuHfHmVPV5ezyU1-P72-nZzf5z0RjOVGcNoqhlujK1pXZQuAocHEYOBKa1JXpFKUKaYJZ4ZzEJTWVDRNI0Bgw-koO_n17YN_XUFMcmmjhq5TDvwqSsLrmvIhlHJAj_-hC78KbvhOUlyIqmCUFAOFf6k328G7HBJcqvAucSHXRch1EXJThLybPjxuLvoDxBuAjw</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Altarazi, Ahmed</creator><creator>Jadaan, Layali</creator><creator>McBain, Andrew J.</creator><creator>Haider, Julfikar</creator><creator>Kushnerev, Evgeny</creator><creator>Yates, Julian M.</creator><creator>Alhotan, Abdulaziz</creator><creator>Silikas, Nick</creator><creator>Devlin, Hugh</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0537-8884</orcidid><orcidid>https://orcid.org/0000-0001-7010-8285</orcidid></search><sort><creationdate>202409</creationdate><title>3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans</title><author>Altarazi, Ahmed ; Jadaan, Layali ; McBain, Andrew J. ; Haider, Julfikar ; Kushnerev, Evgeny ; Yates, Julian M. ; Alhotan, Abdulaziz ; Silikas, Nick ; Devlin, Hugh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2944-f983da41dfc63765dee1eb12f1e8acc27626a34a4c284f88e933739bbb9e91f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>additive manufacturing</topic><topic>antifungal</topic><topic>Antifungal activity</topic><topic>Biocompatibility</topic><topic>Candida albicans</topic><topic>Cell proliferation</topic><topic>Composite materials</topic><topic>Cytotoxicity</topic><topic>denture base resin</topic><topic>Dentures</topic><topic>Fourier transforms</topic><topic>L-Lactate dehydrogenase</topic><topic>Nanocomposites</topic><topic>Nanoparticles</topic><topic>Resins</topic><topic>Titanium dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Altarazi, Ahmed</creatorcontrib><creatorcontrib>Jadaan, Layali</creatorcontrib><creatorcontrib>McBain, Andrew J.</creatorcontrib><creatorcontrib>Haider, Julfikar</creatorcontrib><creatorcontrib>Kushnerev, Evgeny</creatorcontrib><creatorcontrib>Yates, Julian M.</creatorcontrib><creatorcontrib>Alhotan, Abdulaziz</creatorcontrib><creatorcontrib>Silikas, Nick</creatorcontrib><creatorcontrib>Devlin, Hugh</creatorcontrib><collection>Wiley Online Library (Open Access Collection)</collection><collection>Wiley Online Library Free Content</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of prosthodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Altarazi, Ahmed</au><au>Jadaan, Layali</au><au>McBain, Andrew J.</au><au>Haider, Julfikar</au><au>Kushnerev, Evgeny</au><au>Yates, Julian M.</au><au>Alhotan, Abdulaziz</au><au>Silikas, Nick</au><au>Devlin, Hugh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans</atitle><jtitle>Journal of prosthodontics</jtitle><date>2024-09</date><risdate>2024</risdate><volume>33</volume><issue>S1</issue><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>1059-941X</issn><issn>1532-849X</issn><eissn>1532-849X</eissn><abstract>Purpose
To develop a biocompatible denture base resin/TiO2 nanocomposite material with antifungal characteristics that is suitable for 3D‐printing denture bases.
Materials and Methods
TiO2 nanoparticles (NPs) with a 0.10, 0.25, 0.50, and 0.75 weight percent (wt.%) were incorporated into a commercially available 3D‐printed resin material. The resulting nanocomposite material was analyzed using Lactate dehydrogenase (LDH) and AlamarBlue (AB) assays for biocompatibility testing with human gingival fibroblasts (HGF). The composite material was also tested for its antifungal efficacy against Candida albicans. Fourier transform infrared (FTIR) and Energy Dispersive X‐ray Spectroscopy (EDX) mapping were conducted to assess the surface coating and the dispersion of the NPs.
Results
LDH and AB assays confirmed the biocompatibility of the material showing cell proliferation at a rate of nearly 100% at day 10, with a cytotoxicity of less than 13% of the cells at day 10. The concentrations of 0.10, 0.25, and 0.50 wt.% caused a significant reduction (p < 0.05) in the number of candida cells attached to the surface of the specimens (p < 0.05), while 0.75 wt.% did not show any significant difference compared to the control (no TiO2 NPs) (p > 0.05). FTIR and EDX analysis confirmed the presence of TiO2 NPs within the nanocomposite material with a homogenous dispersion for 0.10 and 0.25 wt.% groups and an aggregation of the NPs within the material at higher concentrations.
Conclusion
The addition of TiO2 NPs into 3D‐printed denture base resin proved to have an antifungal effect against Candida albicans. The resultant nanocomposite material was a biocompatible material with HGFs and was successfully used for 3D printing.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1111/jopr.13784</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-0537-8884</orcidid><orcidid>https://orcid.org/0000-0001-7010-8285</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | additive manufacturing antifungal Antifungal activity Biocompatibility Candida albicans Cell proliferation Composite materials Cytotoxicity denture base resin Dentures Fourier transforms L-Lactate dehydrogenase Nanocomposites Nanoparticles Resins Titanium dioxide |
| title | 3D‐printed nanocomposite denture base resin: The effect of incorporating TiO2 nanoparticles on the growth of Candida albicans |
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