In-situ microbial protein production by using nitrogen extracted from multifunctional bio-electrochemical system

Upgrading of waste nitrogen sources is considered as an important approach to promote sustainable development. In this study, a multifunctional bio-electrochemical system with three chambers was established, innovatively achieving 2.02 g/L in-situ microbial protein (MP) production via hydrogen-oxidizing bacteria (HOB) in the protein chamber (middle chamber), along with over 2.9 L CO2/(L·d) consumption rate. Also, 69% chemical oxygen demand was degraded by electrogenic bacteria in the anode chamber, resulting in the 394.67 J/L electricity generation. Focusing on the NH4+-N migration in the system, the current intensity contributed 4%–9% in the anode and protein chamber, whereas, the negative effect of −6.69% on contribution was shown in the cathode chamber. On the view of kinetics, NH4+-N migration in anode and cathode chambers was fitted well with Levenberg-Marquardt equation (R2 > 0.92), along with the well-matched results of HOB growth in the protein chamber based on Gompertz model (R2 > 0.99). Further evaluating MPs produced by HOB, 0.45 g/L essential amino acids was detected, showing the better amino acid profile than fish and soybean. Multifunctional bio-electrochemical system revealed the economic potential of producing 6.69 €/m3 wastewater according to a simplified economic evaluation. [Display omitted] •43% of NH4+-N was used for in-situ microbial protein (MP) production (2.02 g/L).•Current intensity contributed 8.25% of NH4+-N migration in the protein chamber.•Degradation efficiency of organics was 69% in the anode chamber.•Fixation rate of over 2.9LCO2/(L·d) was obtained, with energy production of 395J/L.•Essential amino acid of 0.46 g/L was gained in MP, better than fish and soybeans.